MUTAGENESIS OF XERODERMA PIGMENTOSUM FIBROBLASTS BY ACROLEIN
MUTAGENESIS OF XERODERMA PIGMENTOSUM FIBROBLASTS BY ACROLEIN
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DOI：
10.1016/0165-7992(88)90104-2
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发表时间：
1988-09-01
期刊：
影响因子：
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通讯作者：
HARRIS, CC
中科院分区：
文献类型：
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作者：
CURREN, RD;YANG, LL;HARRIS, CC
丙烯醛是一种短链醛，作为烟草烟雾的成分和普遍存在的环境污染物，测试了其对人成纤维细胞的毒性和致突变作用。我们发现以DNA修复缺陷为特征的人细胞（来自着色性干皮病（XP）患者的细胞）敏感得多（D37. 0.25μ M）对丙烯醛的细胞毒性作用的影响比来自正常个体的细胞（D37 simeq. 0.8μ M）。丙烯醛对XP细胞也具有强烈的致突变性（在0.2和0.8 μ M丙烯醛之间观察到剂量反应）;然而，丙烯醛并没有诱导正常成纤维细胞突变频率的增加。这种明显缺乏正常人体细胞的致突变性的可能原因进行了讨论。
Acrolein, a short-chain aldehyde encountered as a component of tobacco smoke and as a ubiquitous environmental contaminant, was tested for its toxic and mutagenic effects toward human fibroblast cells. We found that human cells characterized by a deficiency in DNA repair (cells from xeroderma pigmentosum (XP) patients) were much more sensitive (D37 .simeq. 0.25 .mu.M) to the cytotoxic effects of acrolein than were cells from normal individuals (D37 .simeq. 0.8 .mu.M). Acrolein was also strongly mutagenic to the XP cells (a dose response was observed between 0.2 and 0.8 .mu.M acrolein); however acrolein did not induce an increase in the mutant frequency of normal fibroblasts. Possible reasons for this apparent lack of mutagenicity in normal human cells are discussed.