Raman-based detection of bacteria using silver nanoparticles conjugated with antibodies
Raman-based detection of bacteria using silver nanoparticles conjugated with antibodies
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DOI：
10.1039/b701160a
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发表时间：
2007-01-01
期刊：
影响因子：
4.2
通讯作者：
Luong, John H. T.
中科院分区：
文献类型：
--
作者：
Naja, Ghinwa;Bouvrette, Pierre;Luong, John H. T.
表面增强拉曼散射（Sers）已被用于检测吸附在蛋白A修饰的银纳米颗粒上的多克隆抗体捕获的细菌。该技术的选择性和歧视，保证通过使用一个特定的抗体的模式细菌，大肠杆菌。由于Sers增强机制取决于金属表面的接近程度，因此认为细菌与纳米颗粒表面之间的最佳距离为8 nm。使用主成分分析来验证光谱再现性，以区分与吸附到纳米颗粒上的生物分子和/或细菌相对应的簇。与普通的拉曼光谱相比，Sers技术导致超过20倍的强度增强。
Surface enhanced Raman scattering (SERS) has been used to detect bacteria captured by polyclonal antibodies sorbed onto protein-A-modified silver nanoparticles. The selectivity and discrimination of the technique were assured by using a specific antibody to the model bacterium, Escherichia coli. As the SERS enhancement mechanism depends upon the metal surface proximity, 8 nm was considered as the optimum distance between the bacterium and the nanoparticle surface. Spectral reproducibility was verified using Principal Components Analysis to differentiate the clusters corresponding to the biomolecules and/or bacteria sorbed onto nanoparticles. Compared to the normal Raman spectrum, the SERS technique resulted in an intensity enhancement of over 20-fold.