Expression of protein kinase-C substrate mRNA in the motor cortex of adult and infant macaque monkeys
Expression of protein kinase-C substrate mRNA in the motor cortex of adult and infant macaque monkeys
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DOI：
10.1016/j.brainres.2007.07.054
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发表时间：
2007-09-26
期刊：
影响因子：
2.9
通讯作者：
Hayashi, Motoharu
中科院分区：
文献类型：
--
作者：
Higo, Noriyuki;Oishi, Takao;Hayashi, Motoharu
为了了解灵长类动物运动皮层可塑性的分子和细胞基础，我们研究了三种蛋白激酶C（PKC）底物的表达：GAP-43，肉豆蔻酰化富含丙氨酸的C-激酶底物（MARCKS）和神经颗粒素，这是调节突触可塑性的关键分子。三种mRNA的显著信号主要在锥体细胞中观察到。大锥体细胞在第V层，从下行运动道起源，GAP-43和神经颗粒蛋白mRNA的杂交信号比小锥体细胞。我们还进行了双标记原位杂交显示，GAP-43和神经颗粒蛋白的mRNA表达在MARCKS阳性神经元的一个子集。定量分析表明，表达层之间是不同的：第VI层包含最强的和第II层的所有三个mRNA的最弱的信号。GAP-43和MARCKS mRNA在第V层的表达水平高于第III层，而神经颗粒素mRNA在第V层和第III层的表达水平基本相同。从新生儿到成人的发育分析表明，这三种mRNA的表达水平在婴儿运动皮层高于成人。GAP-43和神经颗粒素mRNA的表达在出生后几个月内短暂增加。目前的研究表明，表达的三个PKC底物是特定的细胞类型，皮层层，和出生后的发育阶段。这种特异性表达可能反映了婴儿和成人运动皮层可塑性的功能特化。(c)2007 Elsevier B. V.保留所有权利。
To understand the molecular and cellular bases of plasticity in the primate motor cortex, we investigated the expression of three protein kinase-C (PKC) substrates: GAP-43, myristoylated alanine-rich C-kinase substrate (MARCKS), and neurogranin, which are key molecules regulating synaptic plasticity. Prominent signals for the three mRNAs were primarily observed in pyramidal cells. Large pyramidal cells in layer V, from which the descending motor tract originates, contained weaker hybridization signals for GAP-43 and neurogranin mRNAs than did the smaller pyramidal cells. We also performed double-label in situ hybridization showing that GAP-43 and neurogranin mRNAs were expressed in a subset of MARCKS-positive neurons. Quantitative analysis showed that the expression was different between the layers: layer VI contained the strongest and layer II the weakest signals for all three mRNAs. The expression levels of GAP-43 and MARCKS mRNA in layer V were higher than in layer III, while the expression level of neurogranin mRNA in layer V was almost the same as in layer III. Developmental analysis from the newborn to adult indicated that the expression levels of the three mRNAs were higher in the infant motor cortex than in the adult. The expression of both GAP-43 and neurogranin mRNAs transiently increased over several months postnatally. The present study showed that the expression of the three PKC substrates was specific to cell types, cortical layers, and postnatal developmental stage. The specific expression may reflect functional specialization for plasticity in the motor cortex of both infants and adults. (c) 2007 Elsevier B.V. All rights reserved.