Real-time imaging of type III secretion:: Salmonella SipA injection into host cells
Real-time imaging of type III secretion:: Salmonella SipA injection into host cells
复制标题
DOI：
10.1073/pnas.0503407102
复制
发表时间：
2005-08-30
影响因子：
11.1
通讯作者：
Hardt, WD
中科院分区：
文献类型：
--
作者：
Schlumberger, MC;Müller, AJ;Hardt, WD
许多致病性和共生革兰氏阴性细菌利用 III 型分泌系统将“效应”蛋白注入真核宿主细胞中。这些效应器操纵信号通路来启动共生或疾病。通过使用延时显微镜，我们对沙门氏菌 III 型效应蛋白 SipA/SspA 进入动物细胞的实时成像。 SipA 递送大多在对接后 10-90 秒开始，并持续 100-600 秒，直到细菌 SipA 池（6 +/- 3 x 10(3) 分子）耗尽。对效应蛋白 SopE 也进行了类似的观察。这种 III 型分泌的实时可视化解释了通过这种毒力系统操纵宿主细胞的效率。
Many pathogenic and symbiotic Gram-negative bacteria employ type III secretion systems to inject "effector" proteins into eukaryotic host cells. These effectors manipulate signaling pathways to initiate symbiosis or disease. By using time-lapse microscopy, we have imaged delivery of the Salmonella type III effector protein SipA/SspA into animal cells in real time. SipA delivery mostly began 10-90 sec after docking and proceeded for 100-600 sec until the bacterial SipA pool (6 +/- 3 x 10(3) molecules) was exhausted. Similar observations were made for the effector protein SopE. This visualization of type III secretion in real time explains the efficiency of host cell manipulation by means of this virulence system.