A New Image Analysis Method Based on Morphometric and Fractal Parameters for Rapid Evaluation of In Situ Mammalian Mast Cell Status.
A New Image Analysis Method Based on Morphometric and Fractal Parameters for Rapid Evaluation of In Situ Mammalian Mast Cell Status.
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DOI：
10.1017/s1431927615015342
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发表时间：
2015-12
影响因子：
2.8
通讯作者：
Oskeritzian, Carole A.
中科院分区：
文献类型：
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作者：
Wedman, Piper;Aladhami, Ahmed;Beste, Mary;Edwards, Morgan K.;Chumanevich, Alena;Fuseler, John W.;Oskeritzian, Carole A.
除了其在过敏性疾病中的效应子功能外，组织居住的肥大细胞（MC）还通过其独特的能力来分泌许多生物活性分子在细胞质颗粒中藏有炎症事件的引发者。激活触发了介体通过称为脱粒的调节胞吐作用释放。 MC激活仍通过测量系统限制介质的全身水平来证实。但是，确定MC激活的解剖位置对于疾病诊断很有价值。我们设计了一种基于甲基蓝色（MB）染色的正常小鼠皮肤组织切片的图像分析的计算机辅助形态学方法，该方法可以量化实际的原位MC激活状态。我们认为MC细胞质可以被视为基于激活状态的独特相对质量值的对象。完整和脱粒的MC之间的综合光密度和面积（A）比率显着差异（p <0.001）。分形特征的检查是癌症的转化诊断/预后价值，并且很容易地用于量化细胞骨架形态和脉管系统。分形维度（D）是其比较空间填充能力和结构密度的度量，在完整和脱粒的MC之间也有显着差异（p <0.001）。形态分析提供了一种可靠且可重复的方法，用于原位量化MC激活状态。
Apart from their effector functions in allergic disorders, tissue-resident mast cells (MC) are gaining recognition as initiators of inflammatory events through their distinctive ability to secrete many bioactive molecules harbored in cytoplasmic granules. Activation triggers mediator release through a regulated exocytosis named degranulation. MC activation is still substantiated by measuring systemic levels of MC-restricted mediators. However, identifying the anatomical location of MC activation is valuable for disease diagnosis. We designed a computer-assisted morphometric method based on image analysis of methylene blue (MB)-stained normal mouse skin tissue sections that quantitates actual in situ MC activation status. We reasoned MC cytoplasm could be viewed as an object featuring unique relative mass values based on activation status. Integrated optical density and area (A) ratios were significantly different between intact and degranulated MC (p < 0.001). The examination of fractal characteristics is of translational diagnostic/prognostic value in cancer and readily applied to quantify cytoskeleton morphology and vasculature. Fractal dimension (D), a measure of their comparative space filling capacity and structural density, also differed significantly between intact and degranulated MC (p < 0.001). Morphometric analysis provides a reliable and reproducible method for in situ quantification of MC activation status.