Mannose, mannitol, fructose and 1,5-anhydroglucitol concentrations measured by gas chromatography mass spectrometry in blood plasma of diabetic patients
Mannose, mannitol, fructose and 1,5-anhydroglucitol concentrations measured by gas chromatography mass spectrometry in blood plasma of diabetic patients
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DOI：
10.1016/0009-8981(96)06284-5
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发表时间：
1996-07-15
影响因子：
5
通讯作者：
Pitkanen, E
中科院分区：
文献类型：
--
作者：
Pitkanen, E
应用气相色谱/质谱法测定了糖尿病（DM）患者血浆中的糖。同位素稀释技术用于计算1，5-脱水-D-葡萄糖醇（1，5-AG），而样品的还原氘化和还原产物的回归分析用于计算甘露糖、果糖和甘露醇的浓度。胰岛素依赖型（IDDM; r = 0.74，P = 0.001）和非胰岛素依赖型（NIDDM; r = 0.89，P = 0.001）DM患者甘露糖和葡萄糖浓度均呈正相关。在从血糖浓度波动较大的患者中采集的系列样本中也发现了密切相关性。NIDDM组甘露糖/葡萄糖比值升高（P = 0.007）。两型DM患者血清1，5-AG浓度均降低，但以IDDM患者降低更为明显。NIDDM患者血浆中糖蛋白浓度与血糖浓度呈负相关（r = 0.71，P = 0.02），与糖化血红蛋白（HbA 1c）呈负相关（r = 0.84，P = 0.001）。据推测，甘露糖和葡萄糖通过与肾小管糖载体位点的1，5-AG竞争，导致1，5-脱水葡萄糖醇的高尿排泄，导致糖尿病生物体中的糖耗尽。循环甘露糖的高浓度进一步表明，应检查甘露糖对高血糖症不良反应的作用。研究表明，同位素稀释和还原氘化技术的并行使用在生物体液中单糖的分析中是非常有用的。
Gas chromatography/mass fragmentography was applied to measure sugars in the plasma of patients with diabetes mellitus (DM). The isotope-dilution technique was used in the calculation of 1,5-anhydro-D-glucitol (1,5-AG), whereas reductive deuterization of the samples and regression analysis of the reduction products were used to calculate the concentrations of mannose, fructose and mannitol. The concentrations of mannose and glucose were closely and positively correlated both in insulin-dependent (IDDM; r = 0.74, P = 0.001) and non-insulin-dependent (NIDDM; r = 0.89, P = 0.001) DM. The close correlation was also encountered in serial samples taken from patients with widely fluctuating plasma glucose concentrations. The mannose/glucose ratio was increased in NIDDM (P = 0.007). The concentration of 1,5-AG was decreased in both types of DM, but more markedly in IDDM. The concentration was negatively correlated with glucose concentration (r = 0.71, P = 0.02) and HbA(1c) (r = 0.84, P = 0.001) in NIDDM. It was postulated that both mannose and glucose, by competing with 1,5-AG of renal tubular sugar carrier sites, contribute to the high urinary excretion of 1,5-anhydroglucitol leading to depletion of the sugar in the diabetic organism. The high concentrations of circulating mannose suggested further that the contribution of mannose to the adverse effects of hyperglycaemia should be examined. The study demonstrated that parallel use of the isotope-dilution and reductive deuterization techniques is quite useful in the analysis of monosaccharides in biological fluids.