Multiple roles for laccase2 in butterfly wing pigmentation, scale development, and cuticle tanning
Multiple roles for laccase2 in butterfly wing pigmentation, scale development, and cuticle tanning
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DOI：
10.1111/ede.12338
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发表时间：
2020-07-01
影响因子：
2.9
通讯作者：
Reed, Robert D.
中科院分区：
文献类型：
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作者：
Peng, Ceili L.;Mazo-Vargas, Anyi;Reed, Robert D.
鳞翅目翅鳞在许多功能中发挥着重要作用，包括颜色图案和温度调节。然而，尽管翅膀鳞片很重要，但我们对鳞片图案、发育和着色的遗传机制仍然了解有限。在这里，我们探讨了酚氧化酶编码基因laccase2在若虫科蝴蝶Vanessa cardui翅膀和鳞片发育中的功能。通过 CRISPR/Cas9 基因组编辑生成的漆酶 2 体细胞缺失嵌合体呈现出几种不同的突变表型。与其他非鳞翅目昆虫群体的研究一致，我们观察到黑色素沉着减少和角质层形成缺陷。然而，我们也惊讶地发现对鳞片发育的明显影响，包括翅膀鳞片的完全丧失。这项研究强调了laccase2作为一个在翅膀和鳞片发育中发挥多种作用的基因，并为鳞翅目翅膀颜色的进化提供了新的见解。
Lepidopteran wing scales play important roles in a number of functions including color patterning and thermoregulation. Despite the importance of wing scales, however, we still have a limited understanding of the genetic mechanisms that underlie scale patterning, development, and coloration. Here, we explore the function of the phenoloxidase-encoding genelaccase2in wing and scale development in the nymphalid butterflyVanessa cardui. Somatic deletion mosaics oflaccase2generated by CRISPR/Cas9 genome editing presented several distinct mutant phenotypes. Consistent with the work in other nonlepidopteran insect groups, we observed reductions in melanin pigmentation and defects in cuticle formation. We were also surprised, however, to see distinct effects on scale development including complete loss of wing scales. This study highlightslaccase2as a gene that plays multiple roles in wing and scale development and provides new insight into the evolution of lepidopteran wing coloration.