Modeling and optimization of lipase-catalyzed synthesis of phytosteryl esters of oleic acid by response surface methodology
Modeling and optimization of lipase-catalyzed synthesis of phytosteryl esters of oleic acid by response surface methodology
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DOI：
10.1016/j.foodchem.2006.05.025
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发表时间：
2007-01-01
期刊：
影响因子：
8.8
通讯作者：
Akoh, Casimir C.
中科院分区：
文献类型：
--
作者：
Kim, Byung Hee;Akoh, Casimir C.
在己烷中进行植物甾醇与油酸的酶促酯化以产生植物甾醇酯。响应面法用于模拟反应。皱褶假丝酵母脂肪酶是该反应的生物催化剂。研究的反应因素为温度（Te = 35-55 ℃）、反应时间（t = 4-24 h）、底物摩尔比（Sr = 1-3、油酸：植物甾醇）。酶用量（En = 2-10%）。采用后向消去法进行回归分析，建立了酯化度的二次多项式回归模型，并通过卡方检验进行了验证。所有因素的调查积极影响DE，与T具有最大的影响，其次是En，Sr和Te。t、Sr和En的二次项对DE呈负影响，而Te的二次项对DE无影响。最佳反应条件为：Te，51.3 ℃，t，17.0 h，Sr，2.在此条件下，E_n为7.2%，DE为97.0mol%。(c)2006爱思唯尔有限公司保留所有权利。
Enzymatic esterification of phytosterols with oleic acid to produce phytosteryl esters was performed in hexane. Response surface methodology was used to model the reaction. Candida rugosa lipase was the biocatalyst for the reaction. The reaction factors investigated were temperature (Te = 35-55 degrees C, reaction time (t = 4-24 h), substrate molar ratio (Sr = 1-3, oleic acid: phyto sterols). and enzyme amount (En = 2-10%). Well-fitting quadratic polynomial regression model for degree of esterification (DE) was established after regression analysis with backward elimination and verified by a chi(2) test. All factors investigated positively affected DE, with t having the greatest effect followed by En, Sr, and Te. The quadratic terms of t, Sr, and En showed negative effects on DE, whereas, that of Te had no effect on DE. Optimal reaction conditions were: Te, 51.3 degrees C; t, 17.0 h; Sr, 2. 1; En, 7.2% and DE was 97.0 mol% under these conditions. (c) 2006 Elsevier Ltd. All rights reserved.