Fugu (Takifugu rubripes) serum GlcNAc-binding lectin is a kalliklectin but has different properties from those of a reported homologue.
Fugu (Takifugu rubripes) serum GlcNAc-binding lectin is a kalliklectin but has different properties from those of a reported homologue.
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红鳍东方鲀 (Takifugu rubripes) 血清 GlcNAc 结合凝集素是一种激肽凝集素，但具有与报道的同系物不同的特性。
DOI：
10.1093/jb/mvv033
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Nakamura O
中科院分区：
文献类型：
--
作者：
Tsutsui S;Yamamura N;Yoshida T;Nakamura O
采用亲和层析和凝胶过滤的方法，从河豚血清中纯化出40 kda的n -乙酰-d-氨基葡萄糖结合凝集素。利用河豚基因组数据库进行的n端氨基酸测序和cDNA克隆表明，该凝集素是一种新的凝集素，其同源性与哺乳动物血浆钾溶酶和凝血因子XI具有结构相似性。钾溶酶是一种在平头鱼中发现的凝集素。这是关于一种钾liklectin的第二篇报道，但河豚钾liklectin在其糖结合谱、亚基间关联和组织分布上与先前鉴定的平头钾liklectin不同。这些发现表明，钾素凝集素在不同的鱼类中具有不同的特性。
A 40-kDa lectin withN-acetyl-d-glucosamine-binding ability was purified from the sera of fugu (Takifugu rubripes) by affinity chromatography and subsequent gel filtration. N-terminal amino acid sequencing,in silicocloning using the fugu genome database and cDNA cloning demonstrated that this lectin is a homologue of kalliklectin, a novel lectin that was previously found in the flathead teleostPlatycephalus indicusand has structural similarity to mammalian plasma kallikreins and coagulation factor XI. This is the second report of a kalliklectin, but the fugu kalliklectin differs in its sugar-binding spectra, intersubunit association and tissue distribution from the previously identified flathead kalliklectin. These findings indicate that kalliklectins vary in properties among fish species.