A NEW AND RAPID METHOD FOR THE ISOLATION AND CULTIVATION OF TUBERCLE BACILLI DIRECTLY FROM THE SPUTUM AND FECES.
A NEW AND RAPID METHOD FOR THE ISOLATION AND CULTIVATION OF TUBERCLE BACILLI DIRECTLY FROM THE SPUTUM AND FECES.
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DOI：
10.1084/jem.21.1.38
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发表时间：
1915-01-01
影响因子：
15.3
通讯作者：
Petroff, S A
中科院分区：
文献类型：
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作者：
Petroff, S A
从几乎所有结核病阶段的 69 份痰标本中获得了 69 份阳性培养物。其中六个样本通过直接显微镜检查呈阴性，但培养物给出了阳性结果。这六个样本曾一度呈结核杆菌阳性。从三十二份粪便样本中分离出十九份阳性培养物。所有这三十二个标本经直接显微镜检查均呈阳性结果，其中一些仅显示少量结核杆菌。六个样本并非没有污染生物，其余七个呈阴性。事实证明，本文提出的方法对于从痰液中分离结核杆菌来说非常简单且准确。从粪便中分离结核杆菌的部分成功可能是由于许多杆菌可能已经死亡。
Sixty-nine positive cultures were obtained from sixty-nine specimens of sputum from practically all stages of tuberculosis. Six of these specimens were negative by direct microscopic examination, but the cultures gave positive findings. These six specimens have been positive for tubercle bacilli at some time. Nineteen positive cultures were isolated from thirty-two specimens of feces. All these thirty-two specimens, upon direct microscopical examination, gave positive findings, some showing only a few tubercle bacilli. Six specimens were not free from contaminating organisms, and the remaining seven were negative. The method presented in this paper has proved very simple and accurate for the isolation of tubercle bacilli from sputum. The partial success in isolating tubercle bacilli from feces may be due to the fact that many of the bacilli may be dead.