Multicolor labeling in developmental gene regulatory network analysis.
Multicolor labeling in developmental gene regulatory network analysis.
复制标题
发育基因调控网络分析中的多色标记。
DOI：
10.1007/978-1-62703-974-1_17
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Angerer,LynneM
中科院分区：
文献类型：
--
作者：
Sethi,AdityaJ;Angerer,RobertC;Angerer,LynneM
海胆胚胎是发育基因调控网络（GRN）分析的重要模型系统。本章描述了在海胆胚胎GRN研究中使用多色荧光原位杂交（FISH）以及FISH和免疫组织化学的结合。本文提出的方法可以应用于各种实验设置，其中需要精确的多基因产物空间分辨率来构建发育GRN。
The sea urchin embryo is an important model system for developmental gene regulatory network (GRN) analysis. This chapter describes the use of multicolor fluorescent in situ hybridization (FISH) as well as a combination of FISH and immunohistochemistry in sea urchin embryonic GRN studies. The methods presented here can be applied to a variety of experimental settings where accurate spatial resolution of multiple gene products is required for constructing a developmental GRN.