Catfish lymphocytes expressing CC41-reactive leukocyte immune-type receptors (LITRs) proliferate in response to Edwardsiella ictaluri infection in vitro
Catfish lymphocytes expressing CC41-reactive leukocyte immune-type receptors (LITRs) proliferate in response to Edwardsiella ictaluri infection in vitro
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DOI：
10.1016/j.dci.2020.103610
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发表时间：
2020-05-01
影响因子：
2.9
通讯作者：
Bengten, Eva
中科院分区：
文献类型：
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作者：
Blackmon, Laura E.;Quiniou, Sylvie M. A.;Bengten, Eva
单克隆抗体（mAb）CC 34和CC 41识别白细胞免疫型受体（LITR）的重叠亚群。mAb CC 34针对克隆TS32.15细胞毒性T细胞系产生，mAb CC 41针对克隆NK细胞系TS10.1产生。在这项研究中，在体外模型的开发，以监测CC 34-和CC 41-反应性细胞响应于爱德华氏菌感染。简言之，从个体鲶鱼中分离头肾白细胞和外周血淋巴细胞（PBL），并分别用CellTrace Violet和CellTrace FarRed染料标记。用E.自体外周血淋巴细胞共培养。然后使用流式细胞术分析合并的细胞培养物。在培养后第2、5和7天，在PBL群体中观察到CC 41染色的显著增加，这表明LITR参与了细胞介导的对E. ictaluri。
Monoclonal antibodies (mAbs) CC34 and CC41 recognize overlapping subsets of leukocyte immune-type receptors (LITRs). The mAb CC34 was raised against the clonal TS32.15 cytotoxic T cell line and the mAb CC41 was raised against the clonal NK cell line TS10.1. In this study, an in vitro model was developed to monitor CC34- and CC41-reactive cells in response to Edwardsiella ictaluri infection. Briefly, head kidney leukocytes and peripheral blood lymphocytes (PBL) were isolated from individual catfish and labeled with CellTrace Violet and CellTrace FarRed dye, respectively. Head kidney-derived macrophages were infected with E. ictaluri and then cocultured with autologous PBL. The combined cell cultures were then analyzed using flow cytometry. A significant increase in CC41 staining was observed in the PBL population at 2, 5 and 7 days after culture, which suggest that LITRs are involved in cell-mediated immunity to E. ictaluri.