Oestrogen receptor β-immunoreactivity in gonadotropin releasing hormone-expressing neurones:: Regulation by oestrogen
Oestrogen receptor β-immunoreactivity in gonadotropin releasing hormone-expressing neurones:: Regulation by oestrogen
复制标题
DOI：
10.1046/j.1365-2826.2001.00708.x
复制
发表时间：
2001-09-01
影响因子：
3.2
通讯作者：
Coen, CW
中科院分区：
文献类型：
--
作者：
Kalló, I;Butler, JA;Coen, CW
用双标记免疫组织化学方法确定雌激素受体的β亚型(ERβ-IR)在含有促性腺激素释放激素(GnRH)的细胞中是否存在免疫反应。在永生化的GnRH细胞系GT1-7中，几乎所有的细胞核都呈ERβ免疫阳性。在去卵巢大鼠视前区，超过一半的GnRH神经元(52.0-63.5%)在核内含有ERβ-IR，较少的神经元(5-10%)显示出特别强的ERP核信号。在GT1-7细胞和GnRH神经元的细胞核中存在ERβ-IR，这与最近关于这些细胞中ERβmRNA的报道是一致的。雌激素治疗减少了可检测到ERβ-IR的GnRH神经元的百分比。GnRH神经元中ERβ信号强度的范围和ERβ信号在GnRH神经元中的出现率与采用明场或荧光技术的双标记免疫组织化学方法相似。这些发现增加了ERβ受体介导雌激素对GnRH神经元的直接作用的可能性。
Double-label immunohistochemistry was employed to establish whether immunoreactivity for the beta subtype of the oestrogen receptor (ER beta -IR) is present in gonadotropin releasing hormone (GnRH)-containing cells. In the immortalized GnRH cell line, GT1-7, almost all nuclei were immunoreactive for ER beta. In the preoptic area of ovariectomized rats, more than one-half of the GnRH neurones (52.0-63.5%) contained ER beta -IR within the nucleus; a smaller proportion of these neurones (5-10%) displayed a particularly intense nuclear signal for ERP. The presence of ER beta -IR in the nuclei of GT1-7 cells and GnRH neurones is consistent with recent reports of ER beta mRNA in these cells. Oestrogen treatment reduced the percentage of GnRH neurones with detectable ER beta -IR. The range of signal intensity for ER beta and the incidence of the ER beta signal in GnRH neurones were comparable following double-label immunohistochemistry involving either bright field or fluorescent techniques. These findings raise the possibility that ER beta receptors mediate direct effects of oestrogen on GnRH neurones.