DISTRIBUTION OF SECRETONEURIN, A PEPTIDE DERIVED FROM SECRETOGRANIN-II, IN RAT-BRAIN - AN IMMUNOCYTOCHEMICAL AND RADIOIMMUNOLOGICAL STUDY
DISTRIBUTION OF SECRETONEURIN, A PEPTIDE DERIVED FROM SECRETOGRANIN-II, IN RAT-BRAIN - AN IMMUNOCYTOCHEMICAL AND RADIOIMMUNOLOGICAL STUDY
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DOI：
10.1016/0306-4522(93)90585-4
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发表时间：
1993-06-01
期刊：
影响因子：
3.3
通讯作者：
WINKLER, H
中科院分区：
文献类型：
--
作者：
MARKSTEINER, J;KIRCHMAIR, R;WINKLER, H
通过免疫细胞化学和放射免疫测定法研究了促泌神经素（一种通过蛋白水解加工从其前体促泌素 II 衍生而来的肽）在大鼠中枢神经系统中的分布，并通过原位杂交与促泌素 II 信使 RNA 的分布进行了比较。使用特定的抗血清，在整个中枢神经系统中观察到明显的纤维染色以及较小程度的周核染色。在几个大脑区域中发现了高密度的免疫反应性纤维和末梢，即外侧隔膜、杏仁核内侧部分、一些内侧丘脑核、下丘脑、缰核、脚间核、蓝斑、孤束核、尾部三叉神经核和脊髓的胶质质。放射免疫测定法测量的定量分布与免疫细胞化学发现的不同免疫反应密度非常一致。该肽的最高浓度存在于下丘脑中，特别是在正中隆起处，并且与浓度最高的神经肽相当。免疫阳性核周体的分布与原位杂交获得的促泌素II信使RNA的分布非常一致。大鼠脑中促泌神经素的表达模式是广泛且独特的，与已建立的化学递质和神经肽部分重叠。这种新的脑肽的功能意义仍有待确定。
The distribution of secretoneurin, a peptide derived from its precursor secretogranin II by proteolytic processing, was studied in the central nervous system of the rat by immunocytochemistry and radioimmunoassay and compared to the distribution of secretogranin II messenger RNA by using in situ hybridization. With a specific antiserum a distinct staining of fibers and to a lesser extent also of perikarya was observed throughout the central nervous system. A high density of immunoreactive fibers and terminals was found in several brain areas, i.e. the lateral septum, the medial parts of the amygdala, some medial thalamic nuclei, the hypothalamus, habenula, nucleus interpeduncularis, locus coeruleus, nucleus tractus solitarii, the substantiae gelatinosae of the caudal trigeminal nucleus and of the spinal cord. The quantitative distribution as measured by a radioimmunoassay agreed well wit the varying densities of immunoreactivity found by immunocytochemistry. The highest concentrations of this peptide were present in the hypothalamus, in particular, in the median eminence and are comparable to those of the most highly concentrated neuropeptides. The distribution of immunopositive perikarya corresponded well with that of secretogranin II messenger RNA obtained by in situ hybridization.The pattern of secretoneurin expression in rat brain was widespread and unique, partially overlapping with established chemical transmitters and neuropeptides. The functional significance of this new brain peptide remains to be established.