Decrease of CD4+CD25highFoxp3+ regulatory T cells and elevation of CD19+BAFF-R+ B cells and soluble ICAM-1 in myasthenia gravis
Decrease of CD4+CD25highFoxp3+ regulatory T cells and elevation of CD19+BAFF-R+ B cells and soluble ICAM-1 in myasthenia gravis
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DOI：
10.1016/j.clim.2007.10.001
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发表时间：
2008-02-01
影响因子：
8.6
通讯作者：
Lu, Jia-Hong
中科院分区：
文献类型：
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作者：
Li, Xiang;Xiao, Bao-Guo;Lu, Jia-Hong
重症肌无力（MG）是由神经肌肉接头处的抗肌肉乙酰胆碱受体（AChR）的T细胞依赖性自身抗体引起的。采用ELISA和流式细胞术检测75例MG患者和50例健康对照者外周血中Th 1、Th 2、Th 3细胞因子、炎性细胞因子和趋化因子sICAM-1水平，分析CD 4（+）和CD 8（+）调节细胞表型及CD 19（+）B细胞BAFF-R表达。MG患者血清和培养上清中IL-2、IL-4、IL-10、IL-13、IFN-γ、TNF-α、TGF-β和sCTLA-4水平与健康对照组无差异。MG患者血清和培养上清中IL-12水平均降低，sICAM-1水平均升高。虽然MG患者和健康对照组的CD 8（+）CD 28（-）和CD 8（+）CD 122（+）调节性T细胞的数量没有差异，但MG患者表现出CD 4（+）CD 25（高）Foxp 3（+）调节性T细胞的减少和CD 19（+）BAFF-R+ B细胞的增加，提示MG患者存在T细胞平衡失调和B细胞成熟激活。(c)2007年爱思唯尔公司All rights reserved.
Myasthenia gravis (MG) is caused by T-cell-dependent autoantibodies against muscle acetylcholine receptors (AChR) at the neuromuscular junction. Here, we adopted ELISA and flow cytometry techniques to measure the levels of Th1, Th2, Th3 cytokines, inflammatory cytokine and chemokine sICAM-1 and to analyze the phenotypes of CD4(+) and CD8(+) regulatory cells as well as the expression of BAFF-R on CD19(+) B cells in peripheral blood from 75 MG patients and 50 healthy controls. There were no differences in the levels of IL-2, IL-4, IL-10, IL-13, IFN-gamma, TNF-alpha, TGF-beta and sCTLA-4 in both sera and culture supernatants between MG patients and healthy controls. The level of IL-12 was decreased in culture supernatants from MG patients, and the level of sICAM-1 was increased in both sera and culture supernatants from MG patients. Although the populations of CD8(+)CD28(-) and CD8(+)CD122(+) regulatory T cells were not different between MG patients and healthy controls, MG patients exhibited the decrease of CD4(+)CD25(high)Foxp3(+) regulatory T cells and the increase of CD19(+)BAFF-R+ B cells, revealing that MG patients should display the dysfunction of T cell balance and the activation of B cell maturation. (c) 2007 Elsevier Inc. All rights reserved.