CELL-INTERACTION BETWEEN COMPARTMENTS ESTABLISHES THE PROXIMAL-DISTAL AXIS OF DROSOPHILA LEGS
CELL-INTERACTION BETWEEN COMPARTMENTS ESTABLISHES THE PROXIMAL-DISTAL AXIS OF DROSOPHILA LEGS
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DOI：
10.1038/372175a0
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发表时间：
1994-11-10
期刊：
影响因子：
64.8
通讯作者：
COHEN, SM
中科院分区：
文献类型：
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作者：
DIAZBENJUMEA, FJ;COHEN, B;COHEN, SM
通过转录因子（5-7）的局部表达，果蝇的附属器原基被细分为区室（1-4）。相邻隔室中的细胞之间的相互作用建立了负责产生空间图案和促进发育中的附属物中的细胞增殖的组织中心（7-9）。hedgehog（hh）在腿后室的局部表达：成虫盘引导无翅（wg）在腹前细胞中表达，引导十肢麻痹（dpp）在背前细胞中表达，靠近前-后室边界（8）;然后wg作用于指定腹侧细胞命运（10-12）并组织腿的背腹轴（13，14）。前-后室边界附近的表达wg的腹侧细胞和背侧细胞之间的相互作用促进了腿中轴的形成（14，15）。在这里，我们表明，对数表达细胞在腹前室和dpp表达细胞在背前室的联合行动激活表达的远端，近端-远端轴形成所需的基因在四肢。这些结果表明，前-后和背-腹间室之间的顺序相互作用建立了肢体的近端-远端轴。
THE appendage primordia of Drosophila are subdivided into compartments(1-4) by the localized expression of transcription factors(5-7). Interaction between cells in adjacent compartments establishes organizing centres responsible for generating spatial pattern and promoting cell proliferation in the developing appendages(7-9). Localized expression of hedgehog (hh) in the posterior compartment of the leg: imaginal disc directs expression of wingless (wg) in ventral-anterior cells and decapentaplegic (dpp) in dorsal-anterior cells near the anterior-posterior compartment boundary(8); wg then acts to specify ventral cell fate(10-12) and to organize the dorsal-ventral axis of the leg(13,14). Interaction between wg-expressing ventral cells and dorsal cells near the anterior-posterior compartment boundary promotes axis formation in the leg(14,15). Here we show that the combined action of log-expressing cells in the ventral-anterior compartment and dpp-expressing cells in the dorsal-anterior compartment activates expression of Distal-less, a gene required for proximal-distal axis formation in the limbs. These results demonstrate that sequential interaction between anterior-posterior and dorsal-ventral compartments establishes the proximal-distal axis of the limbs.