In focus in HCB: new histochemical insights into mammalian gametogenesis.
In focus in HCB: new histochemical insights into mammalian gametogenesis.
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HCB 的焦点：对哺乳动物配子发生的新组织化学见解。
DOI：
10.1007/s00418-022-02083-3
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发表时间：
2022
影响因子：
2.3
通讯作者：
Koji T
中科院分区：
文献类型：
--
作者：
Hishikawa Y;Takizawa T;Koji T
在过去的十年中，细胞，器官和个体的再生科学一直是一个大话题，并继续以更快的速度发展，这意味着它可以通过重新编程分化状态或更严格地通过人工操纵表观基因组来替换受损的细胞和器官。然而，在哺乳动物发育的自然过程中，这样的生命事件应该只由精子和卵子的受精触发，整个过程被称为生殖。因此，哺乳动物配子发生的理解应该是非常重要的，也许通过提供必要的线索来解决当前的各种问题，而且提供高度基础的知识，以正确的方式发展再生科学。配子发生的过程是睾丸和卵巢中一系列有序、连续的事件，由于不同分化阶段细胞的局部封闭，因此分析其调控机制需要组织化学方法，以使我们能够原位分析单个细胞中蛋白质及其转录物的表达。事实上，我们已经表明，在精子发生和卵子发生过程中，遗传和表观遗传因子的表达波动显着，并参与生殖细胞动力学的调节，如增殖，分化和死亡，在一个复杂的方式。例如，仅在生殖细胞凋亡中，我们就遇到了各种细胞类型特异性现象（对于精子发生，Wang et al. 1998; Koji et al. 2001; Dai et al. 2015; Koji and柴田2020;对于卵子发生，Hakuno et al. 1996; Liu et al. 2018）。在这里，我们试图组织一个题为“哺乳动物配子发生的新组织化学见解”的特刊，以促进我们对哺乳动物配子发生相关的生理和病理现象的理解的最新进展。
During the past decade, regeneration science of cells, organs, and individuals has been a big topic and continues to develop at an ever faster pace, implying that it could enable the replacement of damaged cells and organs with regenerated ones or rejuvenation by reprogramming the differentiation states or more strictly by artificial manipulation of epigenomes. However, in the natural process of mammalian development, such life events are supposed to be triggered only by fertilization of sperm and eggs, the whole story of which is known as reproduction. Therefore, the understanding of mammalian gametogenesis should be principally important, perhaps by providing essential clues to solve various current problems, and moreover provide highly basic knowledge to evolve regeneration science in the right way. The process of gametogenesis is a well-ordered and continuous progression of events in the testis and ovary, and the analysis of their regulatory mechanisms woud require histochemical approaches to permit us to analyze the expression of proteins and their transcripts in individual cells in situ, because of closed topographical localization of cells of various differentiation stages. In fact, we have shown that during both spermatogenesis and oogenesis, the expression of genetic and epigenetic factors fluctuates significantly and is involved in the regulation of germ cell kinetics such as proliferation, differentiation, and death in a complicated manner. For example, in germ cell apoptosis alone we encountered a variety of cell-type-specific phenomena (for spermatogenesis, Wang et al. 1998; Koji et al. 2001; Dai et al. 2015; Koji and Shibata 2020; and for oogenesis, Hakuno et al. 1996; Liu et al. 2018). Here, we have attempted to organize a Special Issue entitled “New histochemical insights into mammalian gametogenesis,” to promote our knowledge of recent progress in the understanding of physiological and pathological phenomena related to mammalian gametogenesis.