IDENTIFICATION OF RADIAL GLIAL-CELLS WITHIN THE DEVELOPING MURINE CENTRAL NERVOUS-SYSTEM - STUDIES BASED UPON A NEW IMMUNOHISTOCHEMICAL MARKER
IDENTIFICATION OF RADIAL GLIAL-CELLS WITHIN THE DEVELOPING MURINE CENTRAL NERVOUS-SYSTEM - STUDIES BASED UPON A NEW IMMUNOHISTOCHEMICAL MARKER
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DOI：
10.1016/0165-3806(88)90121-6
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发表时间：
1988-11-01
期刊：
影响因子：
--
通讯作者：
CAVINESS, VS
中科院分区：
文献类型：
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作者：
MISSON, JP;EDWARDS, MA;CAVINESS, VS
单克隆抗体 RC2 通过常规杂交瘤方法在小鼠中产生。 RC2 识别的抗原非常稳定，可以进行适合高分辨率光和电子显微镜分析的醛固定。从胎儿发育的中性管阶段开始，抗体在整个中枢神经系统中描绘出具有放射状双极形态的神经上皮细胞亚群。下降过程延伸至心室边缘，上升过程通过一个或多个末端静脉曲张接触神经胶质限制膜。这些细胞在神经发生期的持续存在使得它们被鉴定为放射状胶质细胞。早在 E9-10 时期，纤维似乎就组织成简单的直束。在胎儿发育后期，这些束在密度和轨迹上显示出明显的区域特异性转变，特别是与大脑皮质生成以及小脑和基底神经节发育相关。双相型持续被 RC2 染色，直到它们在产后消失。在围产期染色双极放射状胶质细胞逐渐丧失的同时，RC2 识别脑壁不同水平的多极细胞形式，这与放射状胶质细胞向星形胶质细胞的转化一致。 RC2 还可以识别早在 E15 时脊髓和小脑中的单极细胞形式，以及从出生之日起就识别海马结构的齿状回中的单极细胞形式。小脑中的单极形式被推断为伯格曼神经胶质细胞的祖细胞。尽管已知伯格曼神经胶质细胞会在成年后持续存在，但这些细胞在出生后第 2 周之后就不会被 RC2 染色。 RC2 识别的抗原的稳健性使该探针成为研究双极放射状胶质细胞在有丝分裂周转期间形态转变的宝贵工具。它还为研究整个径向纤维系统的组织和组织发生作用提供了敏感的染色。
The monoclonal antibody RC2 was generated in mouse by conventional hybridoma methodology. The antigen recognized by RC2 is robust, allowing aldehyde fixation appropriate to high resolution light and electron microscopic analyses. From the neutral tube stage of fetal development the antibody delineates throughout the central nervous system a subpopulation of neuroepithelial cells which have a radial bipolar morphology. A descending process extends to the ventricular margin, and an ascending process contacts the glial limiting membrane by one or more endfeet varicosities. The persistence of these cells through the neurogenetic period allows their identification as radial glial. From as early as E9-10 the fibers appear to be organized in simple straight fascicles. Later in fetal development these fascicles show marked region-specific transformations in density and trajectory, particularly in association with cerebral corticogenesis and with cerebellar and basal ganglia development. The bipolar forms continue to stain with RC2 until they disappear in the psotnatal period. Concurrently with a progressive perinatal loss of stained bipolar radial glia, RC2 identifies multipolar cell forms at various levels of the brain wall, as consistent with the transformation of radial glia into astrocytes. RC2 also recognizes monopolar cell forms in the spinal cord and the cerebellum as early as E15, and in the dentate gyrus of the hippocampal formation from the day of birth. Monopolar forms in the cerebellum are inferred to be progenitors of Bergmann glia. Although Bergmann glia are known to persist in adult life, these cells do not stain with RC2 beyond the 2nd postnatal week. The robustness of the antigen recognized by RC2 makes this probe a valuable tool to study the morphological transformations of the bipolar radial glia during their mitotic turnover. It also provides a sensitive stain for the study of the organization and the histogenetic role of the overall radial fiber system.