Scanning electron microscopy and EDX analysis of exocrine and endocrine gland cells of rat pancreas surface-etched in an oxygen plasma.
Scanning electron microscopy and EDX analysis of exocrine and endocrine gland cells of rat pancreas surface-etched in an oxygen plasma.
复制标题
对氧等离子体表面蚀刻的大鼠胰腺的外分泌和内分泌腺细胞进行扫描电子显微镜和 EDX 分析。
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
S. Seta
中科院分区：
文献类型：
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作者：
M. Yoshino;S. Miyasaka;H. Sato;S. Seta
将在戊二醛和四氧化锇中双重固定并包埋在环氧树脂中的胰腺切成0.5-1微米厚的切片。在通过用射频发生器激发氧气而产生的氧等离子体中对切片进行表面蚀刻。外分泌和内分泌细胞的结构成分进行了形态学研究的二次电子图像模式的扫描电镜。此外，为了识别一些细胞成分，如内分泌颗粒，通过SEM获得的蚀刻表面的形态学图像与TEM中看到的那些进行了比较，使用来自相同组织块和相同细胞水平的连续切片。对于微量分析研究，仅用戊二醛固定组织。用扫描电镜/能谱仪分析了外分泌细胞和内分泌细胞的结构组成。在氧等离子体中表面蚀刻1分钟后，0.5-0.8微米厚的切片在SEM下获得更好的分辨率。细胞内结构，如核膜，核仁，线粒体，粗面内质网和酶原颗粒是容易识别的。此外，细胞器的内部结构，如线粒体的嵴被识别。在连续切片中，细胞内结构的排列方式在扫描电镜和透射电镜中是一致的。在细胞内的核仁、核膜和分泌颗粒等成分中均能清晰地检测到磷、硫和钙的峰。
Pancreas, double-fixed in glutaraldehyde and osmium tetroxide and embedded in epoxy resin was cut into sections 0.5-1 micron thick. The sections were surface-etched in an oxygen plasma produced by exciting oxygen with a radio frequency generator. Structural components of exocrine and endocrine cells were morphologically investigated in the secondary electron image mode of the SEM. Moreover, in order to identify some cell components such as endocrine granules, the morphological image obtained of the etched surface by the SEM were compared with those seen in a TEM, using the serial sections from the same tissue block and at the same cellular level. For a microanalytical investigation, tissues were fixed with glutaraldehyde alone. The structural components of exocrine and endocrine cells were analyzed by SEM/EDX. A better resolution under the SEM was obtained of 0.5-0.8 micron thick sections after surface-etching in an oxygen plasma for 1 minute. Intracellular structures such as nuclear membranes, nucleolus, mitochondria, rough endoplasmic reticulum and zymogen granules were readily identifiable. Moreover, the internal structure of organelles such as cristae of mitochondria was recognized. In the serial sections, the mode of arrangement of intracellular structures in the SEM was well consistent with those in the TEM. The peaks of phosphorus, sulphur and calcium were clearly detected from the intracellular components such as nucleolus, nuclear membranes and secretory granules.