Metal preferences and metallation.
Metal preferences and metallation.
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DOI：
10.1074/jbc.r114.588145
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发表时间：
2014-10-10
期刊：
影响因子：
--
通讯作者：
Robinson NJ
中科院分区：
文献类型：
--
作者：
Foster AW;Osman D;Robinson NJ
大多数金属蛋白的金属结合偏好与其金属需求不匹配。因此，估计30%的金属酶的代谢是由金属递送系统辅助的，其中约25%获得预组装的金属辅因子。剩余的约70%被认为与缓冲金属池中的金属竞争。通过将这些池的相对浓度维持为相应金属络合物的稳定性的反函数来进一步辅助金属络合物。例如，镁酶总是倾向于结合锌，而这些金属在没有金属递送系统的金属酶中占主导地位。因此，在大多数细胞内，锌的缓冲浓度至少比镁低一百万倍。
The metal binding preferences of most metalloproteins do not match their metal requirements. Thus, metallation of an estimated 30% of metalloenzymes is aided by metal delivery systems, with ∼25% acquiring preassembled metal cofactors. The remaining ∼70% are presumed to compete for metals from buffered metal pools. Metallation is further aided by maintaining the relative concentrations of these pools as an inverse function of the stabilities of the respective metal complexes. For example, magnesium enzymes always prefer to bind zinc, and these metals dominate the metalloenzymes without metal delivery systems. Therefore, the buffered concentration of zinc is held at least a million-fold below magnesium inside most cells.