The involvement of Cry1 and Cry2 genes in the regulation of the circadian body temperature rhythm in mice
The involvement of Cry1 and Cry2 genes in the regulation of the circadian body temperature rhythm in mice
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DOI：
10.1152/ajpregu.00395.2004
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发表时间：
2005-01-01
影响因子：
2.8
通讯作者：
Kanosue, K
中科院分区：
文献类型：
--
作者：
Nagashima, K;Matsue, K;Kanosue, K
criptochrome基因（Cry 1和Cry 2）参与控制生物钟的分子机制，缺乏这些基因（Cry 1（-/-）/Cry 2（-/-））的小鼠是行为痴呆。据推测，生物钟调节体温调节的特征，导致体温（T-b）节律。然而，没有直接证据证明这一猜测。我们在这里表明，T-b和产热在Cry 1（-/-）/Cry 2（-/-）小鼠在恒定的黑暗下是无菌的。相反，这两种节律都发生在与自发活动和/或进食相关的明暗周期和/或周期性食物限制下，尽管它们不像野生型小鼠那样健壮。Cry 1（-/-）/Cry 2（-/-）小鼠的产热与T-b之间的关系在任何条件下都是线性和相同的，表明它们的T-b节律是由与活动和进食相关的产热节律决定的。然而，无论产热水平如何，野生型小鼠的T-b在活动期比非活动期维持在相对较高的水平。这些结果表明，体温调节反应是根据昼夜节律时相调节的，Cry基因参与了这一机制。
The criptochrome genes (Cry1 and Cry2) are involved in the molecular mechanism that controls the circadian clock, and mice lacking these genes ( Cry1(-/-)/ Cry2(-/-)) are behaviorally arrhythmic. It has been speculated that the circadian clock modulates the characteristics of thermoregulation, resulting in body temperature (T-b) rhythm. However, there is no direct evidence proving this speculation. We show here that T-b and heat production in Cry1(-/-)/Cry2(-/-) mice are arrhythmic under constant darkness. In contrast, both rhythms occur under a light-dark cycle and/or periodical food restriction linked with spontaneous activity and/or eating, although they are not robust as those in wild-type mice. The relationship between heat production and T-b in Cry1(-/-)/Cry2(-/-) mice is linear and identical under any conditions, indicating that their T-b rhythm is determined by heat production rhythm associated with activity and eating. However, T-b in wild-type mice is maintained at a relatively higher level in the active phase than the inactive phase regardless of the heat production level. These results indicate that the thermoregulatory responses are modulated according to the circadian phase, and the Cry genes are involved in this mechanism.