FORMATION OF VIABLE BUT NONCULTURABLE SALMONELLA DURING STARVATION IN CHEMICALLY-DEFINED SOLUTIONS
FORMATION OF VIABLE BUT NONCULTURABLE SALMONELLA DURING STARVATION IN CHEMICALLY-DEFINED SOLUTIONS
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DOI：
10.1111/j.1472-765x.1995.tb01326.x
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发表时间：
1995-06-01
影响因子：
2.4
通讯作者：
FRANK, JF
中科院分区：
文献类型：
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作者：
CHMIELEWSKI, RAN;FRANK, JF
沙门氏菌在水生环境中处于饥饿状态时，会进入一种可生存但不可培养的状态。本研究确定了这种病原体在化学成分确定的溶液中的饥饿存活，并测试了非选择性富集检测活细胞但不可培养细胞的能力。饥饿的Salm在7 ℃下在7.35 mmol l（-1）磷酸钾缓冲液中培养5周后，可培养性完全丧失，而底物反应性细胞群维持在10000细胞ml（-1）以上。饥饿在21摄氏度和饥饿的盐水溶液或较低浓度的磷酸盐缓冲液导致的可培养的人口的生存时间延长，虽然这个人口低于总的可行人口。即使在35 ℃孵育5 d后，使用乳糖肉汤进行富集也不能使活的但不可培养的细胞复苏。
Salmonella enteritidis enters a viable-but-nonculturable state when exposed to starvation in aquatic environments. This study determined starvation survival of this pathogen in chemically defined solutions and tested the ability of nonselective enrichment to detect viable-but-nonculturable cells. Starvation of Salm. enteritidis at 7 degrees C in 7.35 mmol l(-1) potassium phosphate buffer resulted in complete loss of culturability after 5 weeks with maintenance of a substrate-responsive population of over 10000 cell ml(-1). Starvation at 21 degrees C and starvation in saline solutions or lower concentrations of phosphate buffer resulted in prolonged survival of a culturable population although this population was lower than the total viable population. Enrichment using lactose broth did not allow resuscitation of viable-but-nonculturable cells even after 5 d of incubation at 35 degrees C.