CONTROL OF KINETIC-PROPERTIES OF AMPA RECEPTOR CHANNELS BY NUCLEAR-RNA EDITING
CONTROL OF KINETIC-PROPERTIES OF AMPA RECEPTOR CHANNELS BY NUCLEAR-RNA EDITING
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DOI：
10.1126/science.7992055
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发表时间：
1994-12-09
期刊：
影响因子：
56.9
通讯作者：
SEEBURG, PH
中科院分区：
文献类型：
--
作者：
LOMELI, H;MOSBACHER, J;SEEBURG, PH
AMPA（α-氨基-3-羟基-5-甲基-4-异恶唑丙酸）受体通道介导中枢神经系统兴奋性突触后电流的快成分。位点选择性核RNA编辑控制这些通道的钙渗透性，并且在第二个位点的RNA编辑在这里显示影响大鼠大脑中这些通道的动力学方面。在四种AMPA受体亚基中的三种（GluR-B、GluR-C和GluR-D）中，内含子元件决定了初级转录物中称为R/G位点的位置处的密码子转换（阿加（精氨酸）到GGA（甘氨酸）），该位点紧邻可变剪接模块“翻转"和”翻转“之前。这个位点的编辑程度随着大脑的发育以亚基和剪接形式特有的方式进行，编辑过的通道从脱敏中恢复的速度更快。
AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptor channels mediate the fast component of excitatory postsynaptic currents in the central nervous system. Site-selective nuclear RNA editing controls the calcium permeability of these channels, and RNA editing at a second site is shown here to affect the kinetic aspects of these channels in rat brain. In three of the four AMPA receptor subunits (GluR-B, -C, and -D), intronic elements determine a codon switch (AGA, arginine, to GGA, glycine) in the primary transcripts in a position termed the R/G site, which immediately precedes the alternatively spliced modules ''flip'' and ''flop.'' The extent of editing at this site progresses with brain development in a manner specific for subunit and splice form, and edited channels possess faster recovery rates from desensitization.