Intercalation processes of copper complexes in DNA
Intercalation processes of copper complexes in DNA
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DOI：
10.1093/nar/gkv467
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发表时间：
2015-06-23
影响因子：
14.9
通讯作者：
Cortes-Guzman, Fernando
中科院分区：
文献类型：
--
作者：
Galindo-Murillo, Rodrigo;Carlos Garcia-Ramos, Juan;Cortes-Guzman, Fernando
The family of anticancer complexes that include the transition metal copper known as Casiopeinas (R) shows promising results. Two of these complexes are currently in clinical trials. The interaction of these compounds with DNA has been observed experimentally and several hypotheses regarding the mechanism of action have been developed, and these include the generation of reactive oxygen species, phosphate hydrolysis and/or base-pair intercalation. To advance in the understanding on how these ligands interact with DNA, we present a molecular dynamics study of 21 Casiopeinas with a DNA dodecamer using 10 mu s of simulation time for each compound. All the complexes were manually inserted into theminor groove as the starting point of the simulations. The binding energy of each complex and the observed representative type of interaction between the ligand and the DNA is reported. With this extended sampling time, we found that four of the compounds spontaneously flipped open a base pair and moved inside the resulting cavity and four compounds formed stacking interactions with the terminal base pairs. The complexes that formed the intercalation pocket led to more stable interactions.
