Ocular immune responses. I. Priming of A/J mice in the anterior chamber with azobenzenearsonate-derivatized cells induces second-order-like suppressor T cells.
Ocular immune responses. I. Priming of A/J mice in the anterior chamber with azobenzenearsonate-derivatized cells induces second-order-like suppressor T cells.
复制标题
眼部免疫反应。
DOI：
10.4049/jimmunol.128.4.1753
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发表时间：
1982
影响因子：
4.4
通讯作者：
M. Greene
中科院分区：
文献类型：
--
作者：
R. Wetzig;C. Foster;M. Greene
我们研究了小鼠眼前房(AC)预充后的细胞免疫反应。用偶氮苯磺酸偶联脾细胞(ABA-SC)皮下注射的A/J小鼠在5天后用ABA的重氮盐攻击时，表现出足垫肿胀形式的迟发型超敏反应(DTH)。然而，皮下注射ABA-SC时在前房接种ABA-SC的小鼠对ABA具有耐受性。结膜下接种ABA-SC不耐受；相反，它为DTH做好了准备。在AC免疫耐受的小鼠中，不能检测到大量的抗ABA抗体。AC诱导的耐受是半抗原特异性T细胞介导的抑制的结果。AC启动产生的抑制性T细胞(Ts)抑制免疫反应的传出肢体，并且不携带可检测到的交叉反应独特型(CRI)表面受体。在这些表型和功能方面，AC诱导的Ts不同于静脉注射产生的一阶Ts(Ts1)。引爆。有关免疫豁免和眼前房的结果进行了讨论。
We studied the cellular immune responses to ocular anterior chamber (AC) priming of mice. A/J mice primed subcutaneously with azobenzenearsonate-coupled spleen cells (ABA-SC) manifested delayed-type hypersensitivity (DTH) in the form of footpad swelling when challenged 5 days later with the diazonium salt of ABA. Mice inoculated with ABA-SC in the anterior chamber at the time of subcutaneous priming, however, were tolerant to ABA. Subconjunctival inoculation with ABA-SC did not tolerize; rather it primed for DTH. Antibodies against ABA were not detectable in significant amounts in mice made tolerant by AC inoculation. The AC-induced tolerance was shown to result from hapten-specific T cell-mediated suppression. Suppressor T cells (Ts) arising from AC priming suppressed the efferent limb of the immune response and did not bear detectable cross-reactive idiotype (CRI) surface receptors. In these phenotypic and functional respects, AC-induced Ts differed from first-order Ts (Ts1) that result from i.v. priming. The results are discussed with respect to immune privilege and the anterior chamber of the eye.