Dynamic compression of chondrocyte-seeded fibrin gels: effects on matrix accumulation and mechanical stiffness
Dynamic compression of chondrocyte-seeded fibrin gels: effects on matrix accumulation and mechanical stiffness
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DOI：
10.1016/j.joca.2003.08.009
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发表时间：
2004-02-01
影响因子：
7
通讯作者：
Levenston, ME
中科院分区：
文献类型：
--
作者：
Hunter, CJ;Mouw, JK;Levenston, ME
目的：已经测试了各种策略来指导和控制组织工程化软骨中的基质合成，包括植入之前和之后的构造的机械刺激。设计：软骨细胞接种的纤维蛋白凝胶在无限制的机械压缩下培养10或20天（自由膨胀，10%静态，或10+/-4%在0.1或1 Hz）。在培养期间，监测培养基中亚硝酸盐、sGAG和蛋白水解酶的积累。培养后，凝胶的机械刚度和生化含量（DNA，sGAG，和羟脯氨酸含量和GAG A-二糖组成）进行了assessed.Results：相比自由溶胀条件下，静态压缩的机械刚度或生化含量的凝胶的影响不大。与静态压缩相比，振荡压缩产生更软的凝胶，抑制sGAG和羟脯氨酸在凝胶中的积累，并刺激亚硝酸盐和sGAG在培养基中的积累。在DNA含量和Δ-二糖组合物跨治疗conditions.Conclusions：在这项研究中，振荡压缩抑制软骨细胞在纤维蛋白凝胶中形成软骨样组织。这些结果表明，机械刺激对组织工程软骨的影响可能会有很大的不同支架系统之间。(C)2003年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: Various strategies have been tested to direct and control matrix synthesis in tissue engineered cartilage, including mechanical stimulation of the construct both before and after implantation. This study examined the effects of oscillatory compression on chondrocytes in a fibrin-based tissue engineered cartilage.Design: Chondrocyte-seeded fibrin gels were cultured under unconfined mechanical compression for 10 or 20 days (free-swelling, 10% static, or 10+/-4% at 0.1 or 1 Hz). During the culture period, accumulation of nitrite, sGAG, and proteolytic enzymes in the culture media were monitored. Following culture, the mechanical stiffness and biochemical content of the gels (DNA, sGAG, and hydroxyproline content and GAG A-disaccharide composition) were assessed.Results: Compared to free-swelling conditions, static compression had little effect on the mechanical stiffness or biochemical content of the gels. Compared to static compression, oscillatory compression produced softer gels, inhibited sGAG and hydroxyproline accumulation in the gels, and stimulated accumulation of nitrite and sGAG in the culture media. Minimal differences were observed in DNA content and Delta-disaccharide composition across treatment conditions.Conclusions: In this study, oscillatory compression inhibited formation of cartilage-like tissues by chondrocytes in fibrin gels. These results suggest that the effects of mechanical stimuli on tissue engineered cartilage may vary substantially between different scaffold systems. (C) 2003 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.