EXPRESSION OF INTEGRINS BY HUMAN TROPHOBLAST AND DIFFERENTIAL ADHESION TO LAMININ OR FIBRONECTIN
EXPRESSION OF INTEGRINS BY HUMAN TROPHOBLAST AND DIFFERENTIAL ADHESION TO LAMININ OR FIBRONECTIN
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DOI：
10.1093/oxfordjournals.humrep.a138075
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发表时间：
1993-03-01
影响因子：
6.1
通讯作者：
LOKE, YW
中科院分区：
文献类型：
--
作者：
BURROWS, TD;KING, A;LOKE, YW
胎盘着床的过程包括滋养细胞的一系列转化，从停留在基底膜上的单极上皮（绒毛状滋养细胞）到最终分散成独立细胞（间质滋养细胞）侵入子宫蜕膜的细胞聚集体（滋养细胞柱）。这种组织重塑与细胞外基质成分和细胞基质受体表达的变化有关，最相关的是与层粘连蛋白和纤维连接蛋白结合的整合素家族。在这项研究中，我们通过免疫组织学和流式细胞术显示，层粘连蛋白受体的逐渐丧失伴随着纤维连接蛋白受体的获得，因为滋养细胞从绒毛表型转化为细胞柱，进入外细胞膜群。alpha5、alpha6、beta1和beta4亚基的染色模式表明，滋养细胞表达的整合素主要是alpha5beta1和alpha6beta4异二聚体。我们还表明，分离的滋养细胞在laninin上培养时呈现扁平、无根的表型，但在纤连蛋白上培养时表现出更分散、更有活力的形态。此外，在纤维连接蛋白底物上观察到许多多核巨细胞。我们的数据表明层粘连蛋白和纤维连接蛋白受体的相对表达可能决定了滋养细胞在着床过程中的形态和行为。
The process of placental implantation involves a series of transformations of trophoblast from a single polarized epithelial layer resting on a basement membrane (villous trophoblast), to cellular aggregates (trophoblast columns) which ultimately disperse to invade uterine decidua as individual celts (interstitial trophoblast). Such tissue re-modelling is associated with changes in the constituents of the extracellular matrix and in the expression of matrix receptors by the cells, the most relevant being the family of integrins which bind to laminin and fibronectin. In this study we show, by immunohistology and flow cytometry, a gradual loss of laminin receptors with the concomitant acquisition of fibronectin receptors as trophoblast is transformed from the villous phenotype, through the cell columns, into the extravillous population. The pattern of staining for the alpha5, alpha6, beta1 and beta4 subunits indicates that the integrins expressed by trophoblast are predominantly the alpha5beta1 and the alpha6beta4 heterodimers. We have also shown that isolated trophoblast cells assume a flattened, sessile phenotype when cultured on laninin but exhibit a more spreading, motile morphology when plated on fibronectin. In addition, numerous multinucleated giant cells are observed on a fibronectin substrate. Our data suggest that the relative expression of laminin and fibronectin receptors may determine the morphology and behaviour of trophoblast during the process of implantation.