Sensitive detection of maltose and glucose based on dual enzyme-displayed bacteria electrochemical biosensor
Sensitive detection of maltose and glucose based on dual enzyme-displayed bacteria electrochemical biosensor
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基于双酶展示细菌电化学生物传感器灵敏检测麦芽糖和葡萄糖
DOI：
10.1016/j.bios.2016.07.050
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发表时间：
2017-01-15
影响因子：
12.6
通讯作者：
Shi, Jianguo
中科院分区：
文献类型：
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作者：
Liu, Aihua;Lang, Qiaolin;Shi, Jianguo
将葡萄糖淀粉酶显示菌（ga -细菌）和葡萄糖脱氢酶显示菌（gdh -细菌）共固定在多壁碳纳米管修饰的玻碳电极（GCE）上，构建ga -细菌/ gdh -细菌/MWNTs/GCE生物传感器。通过优化加样量和ga -细菌与gdh -细菌的比例，研制出生物传感器。该传感器具有0.2 ~ 10 mM的宽动态范围和0.1 mM的低检测限（S/N=3）。该传感器对葡萄糖在0.1 ~ 2.0 mM范围内具有良好的线性响应，最低检出限为0.04 mM葡萄糖（S/N=3）。有趣的是，在相同浓度下，葡萄糖的敏感性是麦芽糖的3.75倍。对其他可能的单糖和双糖没有观察到干扰。该生物传感器还具有良好的长期储存稳定性和可重复性。此外，使用gdh -细菌/MWNTs/GCE生物传感器和ga -细菌/ gdh -细菌/MWNTs/GCE生物传感器，可以检测实际样品中的葡萄糖和麦芽糖。因此，所提出的生物传感器能够监测食品生产和发酵过程。(C) 2016 Elsevier B.V.版权所有
Glucoamylase-displayed bacteria (GA-bacteria) and glucose dehydrogenase-displayed bacteria (GDH-bacteria) were co-immobilized on multi-walled carbon nanotubes (MWNTs) modified glassy carbon electrode (GCE) to construct GA-bacteria/GDH-bacteria/MWNTs/GCE biosensor. The biosensor was developed by optimizing the loading amount and the ratio of GA-bacteria to GDH-bacteria. The as prepared biosensor exhibited a wide dynamic range of 0.2-10 mM and a low detection limit of 0.1 mM maltose (S/N=3). The biosensor also had a linear response to glucose in the range of 0.1-2.0 mM and a low detection limit of 0.04 mM glucose (S/N=3). Interestingly, at the same concentration, glucose was 3.75-fold sensitive than that of maltose at the proposed biosensor. No interferences were observed for other possible mono- and disaccharides. The biosensor also demonstrated good long-term storage stability and repeatability. Further, using both GDH-bacteria/MWNTs/GCE biosensor and GA-bacteria/GDH-bacteria/MWNTs/GCE biosensor, glucose and maltose in real samples can be detected. Therefore, the proposed biosensor is capable of monitoring the food manufacturing and fermentation process. (C) 2016 Elsevier B.V. All rights reserved.