Myosin heavy chain expression in zebrafish and slow muscle composition
Myosin heavy chain expression in zebrafish and slow muscle composition
复制标题
DOI：
10.1002/dvdy.20380
复制
发表时间：
2005-07-01
影响因子：
2.5
通讯作者：
Currie, PD
中科院分区：
文献类型：
--
作者：
Bryson-Richardson, R;Daggett, DF;Currie, PD
在斑马鱼胚胎中，两种不同类型的肌纤维已被描述在形成肌节，从地形可分离的前体人口。完全基于与针对哺乳动物和鸡肌球蛋白重链同种型的抗体的交叉反应性，慢收缩肌已被证明完全来自“近轴”成肌细胞，其从脊索侧翼的起源迁移形成单层皮下分化的肌细胞。肌节的其余部分在这种迁移之后分化为被抗快速肌球蛋白重链（MyHC）抗体识别的肌纤维。为了确定明确的分子标记的细胞命运的肌节，我们的特点是基因编码斑马鱼快，慢肌细胞。使用系统发育和表达分析，我们证明，这些基因是决定性的分子标记的慢和快的抽搐的命运。我们还表明，斑马鱼胚胎慢肌共表达慢肌和快肌MyHC亚型，一个属性，他们分享与初级纤维的babroote肌节。(c)2005 Wiley-Liss，Inc.
In the zebrafish embryo, two distinct classes of muscle fibers have been described in the forming myotome that arise from topographically separable precursor populations. Based entirely on cross-reactivity with antibodies raised against mammalian and chick myosin heavy chain isoforms slow twitch muscle has been shown to arise exclusively from "adaxial" myoblasts, which migrate from their origin flanking the notochord to form a single layer of subcutaneous differentiated muscle cells. The remainder of the myotome differentiates behind this migration as muscle fibers recognized by anti-fast myosin heavy chain (MyHC) antibodies. To identify unambiguous molecular markers of cell fate in the myotome, we have characterized genes encoding zebrafish fast and slow MyHC. Using phylogenetic and expression analysis, we demonstrate that these genes are definitive molecular markers of slow and fast twitch fates. We also demonstrate that zebrafish embryonic slow twitch muscle co-expresses both slow and fast twitch MyHC isoforms, a property that they share with primary fibers of the amniote myotome. (c) 2005 Wiley-Liss, Inc.