Loss of peroxisomal enzyme expression in preneoplastic and neoplastic lesions induced by peroxisome proliferators in rat livers.
Loss of peroxisomal enzyme expression in preneoplastic and neoplastic lesions induced by peroxisome proliferators in rat livers.
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DOI：
10.1093/carcin/13.2.265
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发表时间：
1992-02
期刊：
影响因子：
4.7
通讯作者：
Y. Yokoyama;S. Tsuchida;I. Hatayama;K. Satoh;T. Narita;M. Rao;J. Reddy;J. Yamada;T. Suga;K. Sato
中科院分区：
文献类型：
--
作者：
Y. Yokoyama;S. Tsuchida;I. Hatayama;K. Satoh;T. Narita;M. Rao;J. Reddy;J. Yamada;T. Suga;K. Sato
免疫组织化学染色的烯酰CoA水合酶（ECH），过氧化物酶体的关键酶，发现假定的癌前病变诱导的过氧化物酶体增殖剂（PP）氯贝丁酯（0.3%的饮食）给大鼠60周或更长时间，缺乏这种酶，使他们可以被检测为ECH阴性病灶。ECH和其他过氧化物酶体酶如酰基CoA氧化酶、过氧化氢酶和肉毒碱依赖性乙酰转移酶在环丙贝特（饲料中0.025%）、Wy-14，643（0.1%）或BR-931（0.2%）诱导的大多数肝增生结节和肝癌中也不表达或仅弱表达，而在周围肝细胞中被强烈诱导。这些结果表明，ECH和其他过氧化物酶体酶的表达被抑制在假定的癌前病变和肿瘤病变诱导的PP在大鼠肝脏，这些过氧化物酶体酶可能因此被用作阴性标志物。
Immunohistochemical staining of enoyl CoA hydratase (ECH), a key peroxisomal enzyme, revealed that the putative preneoplastic lesions induced in livers by administration of the peroxisome proliferator (PP) clofibrate (0.3% in diet) to rats for 60 weeks or more, lacked this enzyme so that they could be detected as ECH-negative foci. ECH and other peroxisomal enzymes such as acyl CoA oxidase, catalase and carnitine-dependent acetyltransferase were also either not or only weakly expressed in most hepatic hyperplastic nodules and hepatomas induced by ciprofibrate (0.025% in diet), Wy-14,643 (0.1%) or BR-931 (0.2%), while being strongly induced in surrounding hepatocytes. These results indicate that the expression of ECH and other peroxisomal enzymes is repressed in putative preneoplastic and neoplastic lesions induced by PPs in rat livers and that these peroxisomal enzymes might therefore be used as negative markers.