Prolactin secretion by cultured anterior pituitary cells: influence of culture conditions and endocrine status of the pituitary donor
Prolactin secretion by cultured anterior pituitary cells: influence of culture conditions and endocrine status of the pituitary donor
复制标题
培养的垂体前叶细胞分泌催乳素：培养条件和垂体供体内分泌状态的影响
DOI：
10.1016/0303-7207(84)90021-2
复制
发表时间：
1984
影响因子：
4.1
通讯作者：
N. Ben
中科院分区：
文献类型：
--
作者：
M. T. Hoefer;M. Heiman;N. Ben
本文研究了体外培养的垂体前叶细胞在有和无儿茶酚胺存在时催乳素（PRL）的分泌特性。PRL分泌受培养条件如细胞密度、培养时间和短期孵育时间的影响。多巴胺（DA）在生理范围内呈剂量依赖性地抑制PRL的释放，这种抑制作用可被立体特异性DA受体拮抗剂（+）-butaclamol逆转。相反，去甲肾上腺素（NE）对PRL分泌的抑制需要更高的剂量，缺乏特异性。从男性捐助者获得的细胞有最低的基础PRL分泌和DA抑制反应最少，而那些从女性在怀孕后期有最高的基础PRL分泌和DA最敏感。
The characteristics of prolactin (PRL) secretion by cultured anterior pituitary cells in the presence and absence of catecholamines were studied. PRL secretion was markedly influenced by culture conditions such as cell density, culture duration and length of short-term incubation. Dopamine (DA) inhibited PRL release in a dose-dependent manner within a physiological range, and this inhibition was reversed by the stereospecific DA receptor antagonist ( + )-butaclamol. In contrast, the inhibition of PRL secretion by norepinephrine (NE) required much higher doses and lacked specificity. Cells obtained from male donors had the lowest basal PRL secretion and were the least responsive to DA inhibition, whereas those obtained from females in late pregnancy had the highest basal PRL secretion and were the most sensitive to DA.