Rodent facial nerve recovery after selected lesions and repair techniques.
Rodent facial nerve recovery after selected lesions and repair techniques.
复制标题
选定病变和修复技术后的啮齿动物面神经恢复。
DOI：
10.1097/prs.0b013e3181c2a5ea
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发表时间：
2010-01
影响因子：
3.6
通讯作者：
Heaton JT
中科院分区：
文献类型：
--
作者：
Hadlock TA;Kowaleski J;Lo D;Mackinnon SE;Heaton JT
测量啮齿动物的面部运动是研究面神经操作恢复的可靠方法，也是检查异常再生的行为相关性的可靠方法。我们定量比较了三种临床相关神经损伤后触须和眼功能的恢复情况。178只成年大鼠进行面神经操作和测试。在实验组中，左侧面神经被压碎，切断并在神经外膜上修复，或切断并将残端缝合固定成一个管，它们之间有2 mm的间隙。在随后的1-4个月内测量面部恢复情况。分析搅拌回收率的数据。先前开发的标记的共同收缩的上部和面中部区（可能的联带运动标记）也进行了检查。挤压组的动物在25天内恢复了几乎正常的搅拌参数。在早期恢复期间，远端分支挤压组的恢复优于主干挤压组。到第9周，横断/修复组显示出恢复的证据，在整个研究期间呈进一步上升趋势。Enturidine组遵循类似的恢复模式，尽管在研究结论中它们没有保持显著的恢复水平。面神经损伤后，选定组的潜在联带运动标记物增加。啮齿类动物的触须功能恢复在一个可预测的方式后操纵。面神经操作后出现上面部和面中部区域的普遍共同收缩，可能与神经操作后啮齿动物对感觉刺激的异常再生、多末端轴突或超敏反应有关。
Measuring rodent facial movements is a reliable method for studying recovery from facial nerve manipulation, and for examining the behavioral correlates of aberrant regeneration. We quantitatively compared recovery of vibrissal and ocular function following three types of clinically relevant nerve injury. 178 adult rats underwent facial nerve manipulation and testing. In the experimental groups, the left facial nerve was either crushed, transected and repaired epineurially, or transected and the stumps suture-secured into a tube with a 2 mm gap between them. Facial recovery was measured for the ensuing 1–4 months. Data were analyzed for whisking recovery. Previously developed markers of co-contraction of the upper and midfacial zones (possible synkinesis markers) were also examined. Animals in the crush groups recovered nearly normal whisking parameters within 25 days. The distal branch crush group showed improved recovery over the main trunk crush group for several days during early recovery. By week 9, the transection/repair groups showed evidence of recovery that trended further upward throughout the study period. The entubulation groups followed a similar recovery pattern, though they did not maintain significant recovery levels by the study conclusion. Markers of potential synkinesis increased in selected groups following facial nerve injury. Rodent vibrissial function recovers in a predictable fashion following manipulation. Generalized co-contraction of the upper and midfacial zones emerges following facial nerve manipulation, possibly related to aberrant regeneration, polyterminal axons, or hypersensitivity of the rodent to sensory stimuli following nerve manipulation.