INCREASED NUMBER OF NON-DEGRANULATED MAST CELLS IN PANCREATIC DUCTAL ADENOCARCINOMA BUT NOT IN ACUTE PANCREATITIS
INCREASED NUMBER OF NON-DEGRANULATED MAST CELLS IN PANCREATIC DUCTAL ADENOCARCINOMA BUT NOT IN ACUTE PANCREATITIS
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DOI：
10.1177/039463201402700208
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发表时间：
2014-04-01
影响因子：
3.5
通讯作者：
Theoharides, T. C.
中科院分区：
文献类型：
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作者：
Karamitopoulou, E.;Shoni, M.;Theoharides, T. C.
越来越多的证据表明，肿瘤微环境（tumor microenvironment， TME）在肿瘤的生存和转移中起着至关重要的作用。炎症细胞聚集在肿瘤周围，奇怪的是，它们似乎是允许肿瘤生长的。肥大细胞是一种关键的基质细胞，它可以分泌大量的促肿瘤分子和抗肿瘤分子。与急性胰腺炎（AP）相比，我们研究了胰腺导管腺癌（PDAC）中MC的存在和脱肉芽状态。三种不同的检测方法：(a)甲苯胺蓝染色，以及(b)胰蛋白酶和(c) c-kit的免疫组织化学，用于评估PDAC组织（n=7）、来自无肿瘤边缘的未受损伤胰腺组织（n=7）和组织形式AP （n=4）中MC脱粒的数量和程度。与来源于无肿瘤边缘的正常胰腺组织相比，PDAC中三种方法检测到的MC数量均显著增加(p
Increasing evidence indicates that tumor microenvironment (TME) is crucial in tumor survival and metastases. Inflammatory cells accumulate around tumors and strangely appear to be permissive to their growth. One key stroma cell is the mast cell (MC), which can secrete numerous pro- and antitumor molecules. We investigated the presence and degranulation state of MC in pancreatic ductal adenocarcinoma (PDAC) as compared to acute pancreatitis (AP). Three different detection methods: (a) toluidine blue staining, as well as immunohistochemistry for (b) tryptase and (c) c-kit, were utilized to assess the number and extent of degranulation of MC in PDAC tissue (n=7), uninvolved pancreatic tissue derived from tumor-free margins (n=7) and tissue form AP (n=4). The number of MC detected with all three methods was significantly increased in PDAC, as compared to normal pancreatic tissue derived from tumor-free margins (p