Rop plays conserved roles in the reproductive and digestive processes of spider mites
Rop plays conserved roles in the reproductive and digestive processes of spider mites
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Rop 在红蜘蛛的繁殖和消化过程中发挥保守作用
DOI：
10.1111/1744-7917.13103
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发表时间：
2022-09-12
期刊：
影响因子：
4
通讯作者：
Hong，Xiao-Yue
中科院分区：
文献类型：
--
作者：
Xia，Xue;Peng，Chang-Wu;Hong，Xiao-Yue
Rasopposite（Rop）在调节囊泡运输，包括突触传递和一般分泌中起重要作用。Rop的基本作用已被许多生物体在胚胎发生过程中的无效突变产生致死表型的观察所证实。然而，Rop在胚后阶段的影响，特别是在非模式生物中，在很大程度上仍然未知。在这里，我们提供了新的数据，提高我们的理解，在成年人的多个物种ofTetranychusspider螨（阿卡里：Tetranychidae），一类臭名昭著的农业害虫罗普的作用。我们的计算机和实验证据表明，Ropis处于纯化选择下，并且在叶螨属中高度保守。RNA干扰实验表明，Rop是维持正常生殖力所必需的，但对存活没有显著影响。我们进一步证明，敲低的Rop变暗的体色的蜘蛛螨，并阻止排泄的粪便颗粒，这是可能有关的异常排泄的食物垃圾在消化系统中。总的来说，我们的研究结果澄清了囊泡运输相关基因在多Tetranychus物种成年阶段的新功能，并强调了评估各种生物体中必需基因作用的必要性。
Rasopposite (Rop) is known to play an essential role in regulating vesicle trafficking, including synaptic transmission and general secretion. The fundamental roles ofRophave been confirmed by the observation that null mutations in many organisms generate lethal phenotypes during embryogenesis. However, the effects of Rop during the postembryonic stages, especially in non‐model organisms, remain largely unknown. Here, we provide new data that enhance our understanding of Rop's roles in the adults of multiple species ofTetranychusspider mites (Acari: Tetranychidae), a class of notorious agricultural pests. Ourin silicoand experimental evidence demonstrated thatRopis under purifying selection and is highly conserved inTetranychusspp. RNA interference experiments showed thatRopis required for maintaining normal fecundity but has no significant effect on survival. We further demonstrate that knockdown of Rop darkens the body color of spider mites and blocks the excretion of fecal pellets, which is likely to be related to an abnormality in the excretion of food waste in the digestive system. Overall, our findings clarify novel functions of a vesicle trafficking‐related gene in the adult stage of multipleTetranychusspecies and highlight the need to evaluate the roles of essential genes in various organisms.