Structure and Distribution of an Unrecognized Interstitium in Human Tissues
Structure and Distribution of an Unrecognized Interstitium in Human Tissues
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DOI：
10.1038/s41598-018-25732-x
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发表时间：
2018-03-27
影响因子：
4.6
通讯作者：
Theise, Neil D.
中科院分区：
文献类型：
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作者：
Benias, Petros C.;Wells, Rebecca G.;Theise, Neil D.
共聚焦激光显微内镜（pCLE）在内窥镜检查期间提供60-70 μ m深度的人体组织的实时组织学成像。荧光素注射后肝外胆管的pCLE在荧光素填充的窦内表现出网状模式，没有已知的解剖学相关性。固定前冷冻活检组织保留了该结构的解剖结构，证明它是粘膜下层的一部分，是以前未被认识到的充满液体的间隙，引流到淋巴结，并由厚胶原束的复杂网络支撑。这些纤维束的一侧间断地衬有成纤维细胞样细胞，这些细胞用内皮标记物和波形蛋白染色，尽管在纤维束的基质蛋白和周围液体之间存在非常不寻常和广泛的无衬界面。我们在许多组织中观察到类似的结构，这些组织受到间歇性或节律性压迫，包括整个胃肠道和膀胱的粘膜下层、真皮、支气管周围和动脉周围软组织以及筋膜。这些解剖结构在癌症转移、水肿、纤维化和许多或所有组织和器官的机械功能中可能是重要的。总之，我们描述了一个以前未被识别的解剖学和组织学，虽然广泛的，宏观的，充满液体的空间内和组织之间，一个新的扩展和规范的概念，人类的胎盘。
Confocal laser endomicroscopy (pCLE) provides real-time histologic imaging of human tissues at a depth of 60-70 mu m during endoscopy. pCLE of the extrahepatic bile duct after fluorescein injection demonstrated a reticular pattern within fluorescein-filled sinuses that had no known anatomical correlate. Freezing biopsy tissue before fixation preserved the anatomy of this structure, demonstrating that it is part of the submucosa and a previously unappreciated fluid-filled interstitial space, draining to lymph nodes and supported by a complex network of thick collagen bundles. These bundles are intermittently lined on one side by fibroblast-like cells that stain with endothelial markers and vimentin, although there is a highly unusual and extensive unlined interface between the matrix proteins of the bundles and the surrounding fluid. We observed similar structures in numerous tissues that are subject to intermittent or rhythmic compression, including the submucosae of the entire gastrointestinal tract and urinary bladder, the dermis, the peri-bronchial and peri-arterial soft tissues, and fascia. These anatomic structures may be important in cancer metastasis, edema, fibrosis, and mechanical functioning of many or all tissues and organs. In sum, we describe the anatomy and histology of a previously unrecognized, though widespread, macroscopic, fluid-filled space within and between tissues, a novel expansion and specification of the concept of the human interstitium.