A conserved transcript pattern in response to a specialist and a generalist herbivore
A conserved transcript pattern in response to a specialist and a generalist herbivore
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DOI：
10.1105/tpc.104.026120
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发表时间：
2004-11-01
期刊：
影响因子：
11.6
通讯作者：
Farmer, EE
中科院分区：
文献类型：
--
作者：
Reymond, P;Bodenhausen, N;Farmer, EE
在分子水平上，植物对攻击的反应所引起的转录模式揭示了植物如何对侵略者作出反应。这些模式是由造成的物理伤害以及攻击者展示或释放的生物成分形成的。因此，不同类型的攻击生物可能会在宿主中引发不同的转录程序。使用大规模的DNA微阵列，我们的特点是基因表达受损，以及在远端拟南芥叶响应专家昆虫，粉蝶。目前已鉴定出100多个可能参与防御的昆虫应答基因，包括与发病机制、吲哚硫代葡萄糖苷代谢、解毒和细胞存活以及信号转导有关的基因。在这114个基因中，有111个基因在粉蝶的摄食中被诱导，只有3个基因被抑制。在远端叶中的表达模式与本地叶中的表达模式非常相似。野生型和茉莉酸突变体植物，再加上茉莉酸处理的分析表明，粉蝶调节基因表达的67%和84%之间的控制，全部或部分，由茉莉酸途径。这是与增加幼虫的表现冠菌素不敏感1光滑1（coi 1 -1 gl 1）突变体。COI 1和GL 1的独立突变导致幼虫体重增加更快，但GL 1突变对所检测的昆虫响应基因的表达影响相对较小。最后，我们比较了转录模式在蝗虫幼虫的专业P. rafallis和一个多面手昆虫，Spodoptera littoralis。令人惊讶的是，考虑到昆虫唾液组分的复杂性质和物种之间的差异，观察到几乎相同的转录谱。本研究也为进一步研究植物-昆虫互作提供了一个强有力的特征基因集。
Transcript patterns elicited in response to attack reveal, at the molecular level, how plants respond to aggressors. These patterns are fashioned both by inflicted physical damage as well as by biological components displayed or released by the attacker. Different types of attacking organisms might therefore be expected to elicit different transcription programs in the host. Using a large-scale DNA microarray, we characterized gene expression in damaged as well as in distal Arabidopsis thaliana leaves in response to the specialist insect, Pieris rapae. More than 100 insect-responsive genes potentially involved in defense were identified, including genes involved in pathogenesis, indole glucosinolate metabolism, detoxification and cell survival, and signal transduction. Of these 114 genes, 111 were induced in Pieris feeding, and only three were repressed. Expression patterns in distal leaves were markedly similar to those of local leaves. Analysis of wild-type and jasmonate mutant plants, coupled with jasmonate treatment, showed that between 67 and 84% of Pieris-regulated gene expression was controlled, totally or in part, by the jasmonate pathway. This was correlated with increased larval performance on the coronatine insensitive1 glabrous1 (coi1-1 gl1) mutant. Independent mutations in COI1 and GL1 led to a faster larval weight gain, but the gl1 mutation had relatively little effect on the expression of the insect-responsive genes examined. Finally, we compared transcript patterns in Arabidopis in response to larvae of the specialist P. rapae and to a generalist insect, Spodoptera littoralis. Surprisingly, given the complex nature of insect salivary components and reported differences between species, almost identical transcript profiles were observed. This study also provides a robustly characterized gene set for the further investigation of plant-insect interaction.