BRASSINOSTEROIDS ACT AS REGULATORS OF TRACHEARY-ELEMENT DIFFERENTIATION IN ISOLATED ZINNIA MESOPHYLL-CELLS
BRASSINOSTEROIDS ACT AS REGULATORS OF TRACHEARY-ELEMENT DIFFERENTIATION IN ISOLATED ZINNIA MESOPHYLL-CELLS
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DOI：
10.1093/oxfordjournals.pcp.a078163
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发表时间：
1991-10-01
影响因子：
4.9
通讯作者：
SHIBAOKA, H
中科院分区：
文献类型：
--
作者：
IWASAKI, T;SHIBAOKA, H
烯效唑[S-3307;（E）-1-（4-氯苯基）-4，4-二甲基-2-（1，2，4-三唑-1-基）-1-戊烯-3-醇]是一种人工合成的植物生长延缓剂。但当以3.4 μ M的浓度添加到培养基中时，对细胞分裂没有影响。 在烯效唑的存在下，没有观察到TE分化过程的细胞学特征事件，如肌动蛋白丝的聚集，微管的成束或次生壁的局部增厚和木质化。 当在培养开始后36 h内将烯效唑加入培养基中时，烯效唑是有效的。 油菜素类固醇（0.2 nM油菜素或2 μ M高油菜素），但不是赤霉素A3，抵消了烯效唑对TE分化的抑制作用。 当在培养开始后24和30小时之间将它们添加到培养物中时，维生素类固醇是最有效的。 外源油菜素内酯促进TE分化。 有人建议，油菜素类固醇的合成是必不可少的分化成TE的细胞和烯效唑抑制这种分化通过其抑制作用油菜素类固醇的生物合成。
Uniconazole [S-3307; (E)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-ol], a synthetic plant-growth retardant, inhibited the differentiation of isolated mesophyll cells of Zinnia elegans L. into tracheary elements (TEs) but had no effect on cell division when it was added to the culture medium at a concentration of 3.4-mu-M. In the presence of uniconazole, none of the cytological events characteristic of the processes of TE differentiation, such as aggregation of actin filaments, bundling of microtubules or localized thickening and lignification of secondary walls, was observed. Uniconazole was effective when it was added to the medium within 36 h after the start of culture. Brassinosteroids (0.2 nM brassinolide or 2-mu-M homobrassinolide), but not gibberellin A3, counteracted the inhibitory effect of uniconazole on TE differentiation. Brassinosteroids were most effective when they were added to cultures between 24 and 30 h after the start of culture. Exogenously applied brassinosteroids promoted TE differentiation. It is suggested that the synthesis of brassinosteroids is essential for the differentiation of the cells into TEs and that uniconazole inhibits this differentiation through its inhibitory effect on the biosynthesis of brassinosteroids.