TIME FOR COFFEE Represses Accumulation of the MYC2 Transcription Factor to Provide Time-of-Day Regulation of Jasmonate Signaling in Arabidopsis
TIME FOR COFFEE Represses Accumulation of the MYC2 Transcription Factor to Provide Time-of-Day Regulation of Jasmonate Signaling in Arabidopsis
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DOI：
10.1105/tpc.111.095430
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发表时间：
2012-06-01
期刊：
影响因子：
11.6
通讯作者：
Davis, Seth J.
中科院分区：
文献类型：
--
作者：
Shin, Jieun;Heidrich, Katharina;Davis, Seth J.
植物每天都面临着可预测的生物和非生物胁迫，这些胁迫来自昼夜循环。生物钟提供了一种预测机制来响应这些日常压力信号，以增加健康。茉莉酸（JA）是一种植物激素，介导各种生长和胁迫反应。在这里，我们发现，生物钟组件的时间咖啡（TIC）作为一个负因素在JA信号通路。我们发现，tic突变体对JA的生长抑制作用高度敏感，并显示出JA调节的基因表达改变。TIC与JA信号转导的关键转录因子MYC 2相互作用。由此，我们发现生物钟有节奏地调节JA信号。TIC是这个昼夜节律门控过程中的关键决定因素，因此，TIC突变体在对病原体感染的节律性JA反应中存在缺陷。TIC通过抑制MYC 2蛋白的积累和以一种晚上特异性的方式控制CORONATINE INSENSITIVE1的转录抑制来发挥作用。综上所述，我们认为TIC作为昼夜节律振荡器的输出成分直接影响JA信号。
Plants are confronted with predictable daily biotic and abiotic stresses that result from the day-night cycle. The circadian clock provides an anticipation mechanism to respond to these daily stress signals to increase fitness. Jasmonate (JA) is a phytohormone that mediates various growth and stress responses. Here, we found that the circadian-clock component TIME FOR COFFEE (TIC) acts as a negative factor in the JA-signaling pathway. We showed that the tic mutant is hypersensitive to growth-repressive effects of JA and displays altered JA-regulated gene expression. TIC was found to interact with MYC2, a key transcription factor of JA signaling. From this, we discovered that the circadian clock rhythmically regulates JA signaling. TIC is a key determinant in this circadian-gated process, and as a result, the tic mutant is defective in rhythmic JA responses to pathogen infection. TIC acts here by inhibiting MYC2 protein accumulation and by controlling the transcriptional repression of CORONATINE INSENSITIVE1 in an evening-phase-specific manner. Taken together, we propose that TIC acts as an output component of the circadian oscillator to influence JA signaling directly.