Alphacoronavirus Transmissible Gastroenteritis Virus nsp1 Protein Suppresses Protein Translation in Mammalian Cells and in Cell-Free HeLa Cell Extracts but Not in Rabbit Reticulocyte Lysate
Alphacoronavirus Transmissible Gastroenteritis Virus nsp1 Protein Suppresses Protein Translation in Mammalian Cells and in Cell-Free HeLa Cell Extracts but Not in Rabbit Reticulocyte Lysate
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DOI：
10.1128/jvi.01806-10
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发表时间：
2011-01-01
影响因子：
5.4
通讯作者：
Makino, Shinji
中科院分区：
文献类型：
--
作者：
Huang, Cheng;Lokugamage, Kumari G.;Makino, Shinji
传染性胃肠炎病毒（TGEV），一种冠状病毒，nsp 1蛋白，有效地抑制哺乳动物细胞中的蛋白质合成。与严重急性呼吸综合征冠状病毒（一种β冠状病毒）的nsp1蛋白不同，TGEV nsp1蛋白不能结合40 S核糖体亚基或促进宿主mRNA降解。TGEV nsp1也抑制蛋白质翻译在无细胞的HeLa细胞提取物，但是，它不影响翻译在兔网织红细胞裂解物（RRL）。我们的数据表明，HeLa细胞提取物和培养的宿主细胞，而不是RRL，含有一个主机因子（S）是必不可少的TGEV nsp1诱导的翻译抑制。
The nsp1 protein of transmissible gastroenteritis virus (TGEV), an alphacoronavirus, efficiently suppressed protein synthesis in mammalian cells. Unlike the nsp1 protein of severe acute respiratory syndrome coronavirus, a betacoronavirus, the TGEV nsp1 protein was unable to bind 40S ribosomal subunits or promote host mRNA degradation. TGEV nsp1 also suppressed protein translation in cell-free HeLa cell extract; however, it did not affect translation in rabbit reticulocyte lysate (RRL). Our data suggested that HeLa cell extracts and cultured host cells, but not RRL, contain a host factor(s) that is essential for TGEV nsp1-induced translational suppression.