A SNARE Complex Unique to Seed Plants Is Required for Protein Storage Vacuole Biogenesis and Seed Development of Arabidopsis thaliana
A SNARE Complex Unique to Seed Plants Is Required for Protein Storage Vacuole Biogenesis and Seed Development of Arabidopsis thaliana
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DOI：
10.1105/tpc.107.057711
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发表时间：
2008-11-01
期刊：
影响因子：
11.6
通讯作者：
Ueda, Takashi
中科院分区：
文献类型：
--
作者：
Ebine, Kazuo;Okatani, Yusuke;Ueda, Takashi
SNARE复合物是囊泡运输的关键调节剂，执行运输囊泡或细胞器与靶膜之间的膜融合。一个功能性SNARE复合体由四个卷曲螺旋束组成，其中三个由Q-SNARE提供，另一个由R-SNARE提供。拟南芥VAMP 727是一个R-SNARE，其同源物仅存在于种子植物中。我们已经发现VAMP 727与SYP 22/VAM 3（一种Q-SNARE）共定位在与液泡膜密切相关的前液泡区室/内体的亚群上。遗传和生物化学分析，包括检查的协同作用的vamp 727和syp 22突变，蛋白质定位的组织学检查，和免疫共沉淀从拟南芥裂解物表明，VAMP 727形成一个复合物与SYP 22，VTI 11，SYP 51，这种复合物在液泡运输，种子成熟，和液泡生物发生中起着至关重要的作用。我们认为，VAMP 727复合物介导的前液泡室和液泡之间的膜融合，这一过程已经演变为种子发育的一个重要步骤。
The SNARE complex is a key regulator of vesicular traffic, executing membrane fusion between transport vesicles or organelles and target membranes. A functional SNARE complex consists of four coiled-coil helical bundles, three of which are supplied by Q-SNAREs and another from an R-SNARE. Arabidopsis thaliana VAMP727 is an R-SNARE, with homologs only in seed plants. We have found that VAMP727 colocalizes with SYP22/VAM3, a Q- SNARE, on a subpopulation of prevacuolar compartments/endosomes closely associated with the vacuolar membrane. Genetic and biochemical analyses, including examination of a synergistic interaction of vamp727 and syp22 mutations, histological examination of protein localization, and coimmunoprecipitation from Arabidopsis lysates indicate that VAMP727 forms a complex with SYP22, VTI11, and SYP51 and that this complex plays a crucial role in vacuolar transport, seed maturation, and vacuole biogenesis. We suggest that the VAMP727 complex mediates the membrane fusion between the prevacuolar compartment and the vacuole and that this process has evolved as an essential step for seed development.