Altered Airway Microbiota Composition in Patients With Pulmonary Hypertension
Altered Airway Microbiota Composition in Patients With Pulmonary Hypertension
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DOI：
10.1161/hypertensionaha.120.15025
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发表时间：
2020-09
期刊：
影响因子：
8.3
通讯作者：
Chenting Zhang;Tingting Zhang;Wenju Lu;X. Duan;Xiaoyun Luo;Shiyun Liu;Yuqin Chen;Yi Li;Jiyuan Chen;Jing Liao;D. Zhou;Xu Chen;Huazhuo Feng;G. Gu;Tao Wang;Haiyang Tang;A. Makino;N. Zhong;J. Yuan;Kai Yang;Jian Wang
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文献类型：
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作者：
Chenting Zhang;Tingting Zhang;Wenju Lu;X. Duan;Xiaoyun Luo;Shiyun Liu;Yuqin Chen;Yi Li;Jiyuan Chen;Jing Liao;D. Zhou;Xu Chen;Huazhuo Feng;G. Gu;Tao Wang;Haiyang Tang;A. Makino;N. Zhong;J. Yuan;Kai Yang;Jian Wang
补充数字内容可在文本中找到。呼吸道微生物群组成的改变在许多慢性肺部疾病的进展中已被报道，然而，呼吸道微生物群与肺动脉高压（PH）疾病发展之间的相关性和因果关系在很大程度上仍然未知。本研究旨在定义和比较PH患者和对照受试者咽拭子样本中呼吸道微生物群的组成。共招募118例PH患者和79例对照受试者，采集咽拭子样本，对呼吸道微生物组16S核糖体RNA (16S rRNA) V3-V4区进行测序。PH患者的相对丰度与对照组有很大不同。Ace和Sobs指数表明，咽区微生物丰富度值显著高于对照组；而PH患者的群落多样性值明显低于对照组。主成分分析结果表明，两组患者咽部菌群分布情况不同。线性判别分析效应大小也显示，PH患者中链球菌、Lautropia和Ralstonia的比例明显高于对照组。代表微生物基因功能的线性判别分析效应大小输出表明，PH患者与对照组相比，与细菌侵袭上皮细胞、细菌毒素相关的基因增强，而与能量代谢、蛋白质消化吸收和细胞分裂途径相关的基因减弱。总之，我们的研究首次报道了PH患者和对照患者上呼吸道微生物群的系统定义和不同特征。
Supplemental Digital Content is available in the text. Alteration in microbiota composition of respiratory tract has been reported in the progression of many chronic lung diseases, yet, the correlation and causal link between respiratory tract microbiota and the disease development of pulmonary hypertension (PH) remain largely unknown. This study aims to define and compare the respiratory microbiota composition in pharyngeal swab samples between patients with PH and reference subjects. A total of 118 patients with PH and 79 reference subjects were recruited, and the pharyngeal swab samples were collected to sequence the 16S ribosomal RNA (16S rRNA) V3-V4 region of respiratory microbiome. The relative abundances in patients with PH were profoundly different from reference subjects. The Ace and Sobs indexes indicated that the microbiota richness of pharynx value is significantly higher; while the community diversity value is markedly lower in patients with PH, comparing to those of the reference subjects. The microbiota on pharynx showed a different profile between the 2 groups by principal component analysis. The linear discriminant analysis effect size also revealed a significantly higher proportion of Streptococcus, Lautropia, and Ralstonia in patients with PH than reference subjects. The linear discriminant analysis effect size output, which represents the microbial gene functions, suggest genes related to bacterial invasion of epithelial cells, bacterial toxins were enhanced, while genes related to energy metabolism, protein digestion and absorption, and cell division pathways were attenuated in patients with PH versus reference subjects. In summary, our study reports the first systematic definition and divergent profile of the upper respiratory tract microbiota between patients with PH and reference subjects.