Proton-mediated dynamics of the alkaline conformational transition of yeast iso-1-cytochrome c.
Proton-mediated dynamics of the alkaline conformational transition of yeast iso-1-cytochrome c.
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质子介导的酵母 iso-1-细胞色素 c 碱性构象转变动力学。
DOI：
10.1021/ja0494454
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Bowler,BruceE
中科院分区：
文献类型：
--
作者：
Martinez,RobertE;Bowler,BruceE
本文用pH跃变停流法研究了异1-细胞色素M的一个赖氨酸73→组氨酸的碱性构象转变动力学，以探讨引起这种构象转变的可电离“触发”基团的性质。该突变使置换Met 80的配体的pKa从约10.5移动至约6.6，并且除了置换Met 80的配体之外，还暴露了调节该过程的动力学的另外两个可电离基团。结果进行了讨论的电离平衡蛋白质折叠机制的影响。
The kinetics of the alkaline conformational transition of a Lys 73→His variant of iso-1-cytochromechave been investigated using pH jump stopped-flow methods to probe the nature of the ionizable “trigger” group for this conformational change. This mutation moves the pKaof the ligand replacing Met 80 from about 10.5 to approximately 6.6 and has unmasked two other ionizable groups, besides the ligand replacing Met 80, that modulate the kinetics of this process. The results are discussed in terms of the impact of ionization equilibria on protein folding mechanisms.