Transcriptome Changes Reveal the Toxic Mechanism of Cadmium and Lead Combined Exposure on Silk Production and Web-Weaving Behavior of Spider A. ventricosus
Transcriptome Changes Reveal the Toxic Mechanism of Cadmium and Lead Combined Exposure on Silk Production and Web-Weaving Behavior of Spider A. ventricosus
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DOI：
10.1021/acs.est.3c06838
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发表时间：
2023-09-26
影响因子：
11.4
通讯作者：
Lan，Jiao
中科院分区：
文献类型：
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作者：
Yang，Huilin;Yang，Jing;Lan，Jiao
多种金属的联合暴露对生物体的生理生态功能造成了巨大的负担;然而，确切的机制在很大程度上仍然未知。在这里，成年女性。心室暴露于单一和联合暴露于镉（Cd）和铅（Pb）通过食物链。目的是探讨这些金属的组合毒性的丝绸生产和织网行为在生理，细胞形态学和转录水平。Cd、Pb复合暴露显著抑制了丝的产生和结网能力，表现为丝纤维重量和单丝直径减少、结网位置降低、夜间结网和无网次数增加，且Cd、Pb生物积累呈现剂量-反应关系。同时，观察到严重的氧化应激和细胞退行性变化。此外，镉和铅的复合污染表现出协同效应，受浓度变化的影响，富集金属，抑制丝重，氧化损伤和细胞变性。在转录组水平上，上调壶腹腺蛛丝蛋白基因和下调氨基酸合成代谢基因，上调Far基因和下调与细胞凋亡相关的TUBA基因，以及过表达的AChE和Glu基因可能倾向于作为丝蛋白合成、细胞变性和神经递质诱导的生物标志物呈现出有希望的潜力。该研究为全面了解重金属污染的生态毒理效应和机理提供了巨大的能力。
The combined exposure of multiple metals imposes a substantial burden on the ecophysiological functions in organisms; however, the precise mechanism(s) remains largely unknown. Here, adult femaleA. ventricosuswere exposed to single and combined exposure to cadmium (Cd) and lead (Pb) through the food chain. The aim was to explore the combined toxicity of these metals on silk production and web-weaving behavior at physiological, cellular morphological, and transcriptomic levels. The Cd and Pb combined exposure significantly inhibited the ability of silk production and web-weaving, including reduced silk fiber weight and diameter of single strands, lowered weaving position, induced nocturnal weaving, and increased instances of no-web, and showed a dose–response relationship on the Cd and Pb bioaccumulation. Concurrently, severe oxidative stress and degenerative changes in cells were observed. In addition, the combined pollution of Cd and Pb demonstrated synergistic effects, influenced by variations in concentration, on the enrichment of metals, inhibition of silk weight, oxidative damage, and cellular degeneration. At the transcriptome level, the upregulated ampullate spidroin genes and downregulated amino acid anabolic genes, upregulated Far genes and downregulated cytoskeleton-related TUBA genes, and overexpressed AChE and Glu genes may tend to present promising potential as biomarkers for silk protein synthesis, cellular degeneration, and neurotransmitter induction. This study offers an enormous capability for a comprehensive understanding of the eco-toxicological effects and mechanisms of multiheavy metals pollution.