Implantation-dependent expression of trophinin by maternal fallopian tube epithelia during tubal pregnancies - Possible role of human chorionic gonadotrophin on ectopic pregnancy
Implantation-dependent expression of trophinin by maternal fallopian tube epithelia during tubal pregnancies - Possible role of human chorionic gonadotrophin on ectopic pregnancy
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DOI：
10.1016/s0002-9440(10)63579-1
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发表时间：
2003-12-01
影响因子：
6
通讯作者：
Fukuda, MN
中科院分区：
文献类型：
--
作者：
Nakayama, J;Aoki, D;Fukuda, MN
Trophinin、tastin 和 bystin 已被确定为可能参与人类胚胎植入的分子。滋养层细胞和子宫内膜上皮细胞均表达肌营养蛋白，其通过同源肌营养蛋白-肌营养蛋白结合介导顶端细胞粘附。我们假设肌营养蛋白在胚胎植入中的功能是人类独有的，并研究了肌营养蛋白、肌蛋白和Bystin在异位妊娠（一种人类独有的病症）中的表达。在输卵管妊娠中，滋养层细胞和输卵管上皮细胞中均发现这三种物质的含量都很高。母体细胞中肌营养蛋白的表达在邻近滋养细胞的区域特别高，而在子宫内妊娠或未妊娠的女性的完整输卵管中几乎检测不到肌营养蛋白。当完整输卵管外植体与人绒毛膜促性腺激素 (hCG) 一起孵育时，上皮细胞中的滋养蛋白表达增强。由于人类囊胚的滋养外胚层在植入前后都会分泌 hCG，因此这些结果表明，来自人类胚胎的 hCG 诱导母体细胞表达肌营养蛋白。由于 hCG 和肌营养蛋白基因的 β 亚基在哺乳动物中存在差异，本研究表明 hCG 和肌营养蛋白在人类胚胎着床（包括异位妊娠的发病机制）中具有独特的作用。
Trophinin, tastin, and bystin have been identified as molecules potentially involved in human embryo implantation. Both trophoblasts and endometrial epithelial cells express trophinin, which mediates apical cell adhesion through homophilic trophinin-trophinin binding. We hypothesized that trophinin's function in embryo implantation is unique to humans and investigated the expression of trophinin, tastin, and bystin in ectopic pregnancy, a condition unique to humans. in tubal pregnancies, high levels of all three were found in both trophoblasts and fallopian tubal epithelia. Trophinin expression in maternal cells was particularly high in the area adjacent to the trophoblasts, whereas trophinin was barely detectable in intact fallopian tubes from women with in utero pregnancies or without pregnancies. When explants of intact fallopian tube were incubated with the human chorionic gonadotrophin (hCG), trophinin expression was enhanced in epithelial cells. Since the trophectoderm of the human blastocyst secretes hCG before and after implantation, these results suggest that hCG from the human embryo induces trophinin expression by maternal cells. As both beta-subunit of hCG and trophinin genes have diverged in mammals, the present study suggests a unique role of hCG and trophinin in human embryo implantation, including the pathogenesis of ectopic pregnancy.