In vitro screening of traditionally used medicinal plants in China against enteroviruses
In vitro screening of traditionally used medicinal plants in China against enteroviruses
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DOI：
10.3748/wjg.v12.i25.4078
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发表时间：
2006-07-07
影响因子：
4.3
通讯作者：
Li, Jun-Wen
中科院分区：
文献类型：
--
作者：
Guo, Jin-Peng;Pang, Ji;Li, Jun-Wen
目的：目的：从中药中寻找新的抗病毒药物，特别是抗肠道病毒药物.方法：采用MTT比色法测定100多种常用药用植物水提物（AE）的体外抗柯萨奇病毒B3（Coxsackie virus 133）活性.结果：16种植物水提物具有不同程度的抗柯萨奇病毒B3（Coxsackie virus B3）活性.它们可以抑制感染过程中的三个步骤（灭活，吸附和复制）。在16种植物中，大红袍（Sargentodoxacuneata（Oliv.）Rehd.和威尔斯，越南槐，紫苞牡丹Spatholobus suberectus Dunn.和贯众（Cyrtomium fortunei J.sm.）也具有抗其它肠道病毒的活性，包括科萨基病毒135、脊髓灰质炎病毒I、埃可病毒9和埃可病毒29。细胞毒试验表明，所有供试AE的CC_（50）值均高于其EC_（50）值。中国的抗病毒活性较强，其中一些化合物值得进一步研究。(C)2006年，WJG出版社。All rights reserved.
AIM: To search for new antiviral agents from traditional Chinese medicine, specifically anti-enterovirosuses agents.METHODS: The aqueous extracts (AE) of more than 100 traditionally used medicinal plants in China were evaluated for their in vitro anti-Coxsackie virus 133 activities with a MTT-based colorimetric assay.RESULTS: The test for AE of 16 plants exhibited anti-Coxsackie virus B3 activities at different magnitudes of potency. They can inhibit three steps (inactivation, adsorption and replication) during the infection. Among the 16 plants, Sargentodoxa cuneata (Oliv.) Rehd. et Wils., Sophora tonkinensis Gapnep., Paeonia veitchii Lynch, Spatholobus suberectus Dunn. and Cyrtomium fortunei J. sm. also have activity against other enterovirus, including Coxsackie virus 135, Polio virus I, Echo virus 9 and Echo virus 29. Cell cytotoxic assay demonstrated that all tested AE had CC50 values higher than their EC50 values.CONCLUSION: The sixteen traditionally used medicinal plants in. China possessed antiviral activity, and some of them merit further investigations. (C) 2006 The WJG Press. All rights reserved.