Improved bioavailability and biodegradation of a model polyaromatic hydrocarbon by a biosurfactant producing bacterium of marine origin
Improved bioavailability and biodegradation of a model polyaromatic hydrocarbon by a biosurfactant producing bacterium of marine origin
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DOI：
10.1016/j.chemosphere.2008.05.015
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发表时间：
2008-07-01
期刊：
影响因子：
8.8
通讯作者：
Sen, Ramkrishna
中科院分区：
文献类型：
--
作者：
Das, Palashpriya;Mukherjee, Soumen;Sen, Ramkrishna
多环芳烃（PAHs）是一种有机污染物，主要来自化石燃料的加工和燃烧，对人类健康造成危害。在本研究中，使用产生海洋生物表面活性剂的环状芽孢杆菌菌株来提高模型多芳烃蒽的生物利用度和随后的降解。尽管该生物体不能利用蒽作为唯一的碳源，但与正常的甘油矿物盐培养基 (GlyMSM) 相比，它在补充蒽的甘油矿物盐培养基 (AGlyMSM) 中表现出更好的生长和生物表面活性剂生产。该生物表面活性剂产品对各种碳氢化合物表现出高度的乳化作用。气相色谱（GC）、高效薄层色谱（HPTLC）和傅里叶变换红外光谱（FTIR）分析表明，该生物表面活性剂可以有效地截留和溶解PAH。薄层色谱分析表明蒽可用作生产生物表面活性剂的碳底物。因此，有机污染物蒽被代谢并转化为生物表面活性剂，促进其自身的生物修复。 (c) 2008 Elsevier Ltd. 保留所有权利。
Polyaromatic hydrocarbons (PAHs) are organic pollutants mostly derived from the processing and combustion of fossil fuels and cause human health hazards. In the present study a marine biosurfactant producing strain of Bacillus circulans was used to increase the bioavailability and consequent degradation of a model polyaromatic hydrocarbon, anthracene. Although the organism could not utilize anthracene as the sole carbon source, it showed better growth and biosurfactant production in an anthracene supplemented glycerol mineral salts medium (AGlyMSM) compared to a normal glycerol mineral salts medium (GlyMSM). The biosurfactant product showed high degree of emulsification of various hydrocarbons. Analysis by gas chromatography (GC), high performance thin layer chromatography (HPTLC) and Fourier transform infrared spectroscopy (FTIR) showed that the biosurfactant could effectively entrap and solu bilize PAH. Thin layer chromatographic analysis showed that anthracene was utilized as a carbon sub strate for the production of biosurfactant. Thus organic pollutant anthracene was metabolized and converted to biosurfactants facilitating its own bioremediation. (c) 2008 Elsevier Ltd. All rights reserved.