Spatiotemporal control of migration of single cells on a photoactivatable cell microarray
Spatiotemporal control of migration of single cells on a photoactivatable cell microarray
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DOI：
10.1021/ja070294p
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发表时间：
2007-05-30
影响因子：
15
通讯作者：
Maeda, Mizuo
中科院分区：
文献类型：
--
作者：
Nakanishi, Jun;Kikuchi, Yukiko;Maeda, Mizuo
在可光活化的细胞培养基质上实现了细胞迁移的时空控制。单细胞在基板上进行微图案化，并被诱导延伸的片状伪足或丝状伪足导致的突起，或者由随后形成的宽或窄的路径，分别在其周围。通过以微阵列形式跟踪单细胞的迁移，我们对单细胞的迁移率进行了定量分析。
Spatiotemporal control of cell migration was achieved on a photoactivatable cell-culturing substrate. Single cells were micropatterned on the substrate and were induced to extend protrusions led by lamellipodia or filopodia alternatively by the subsequent formation of wide or narrow paths in their surroundings, respectively. By tracking the migration of single cells in a microarray format, we performed quantitative analysis of the migration rates of single cells.