Supplemental diagnosis of a myxozoan parasite from common carp Cyprinus carpio: Synonymy of Thelohanellus xinyangensis with Thelohanellus kitauei
Supplemental diagnosis of a myxozoan parasite from common carp Cyprinus carpio: Synonymy of Thelohanellus xinyangensis with Thelohanellus kitauei
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DOI：
10.1016/j.vetpar.2011.01.008
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发表时间：
2011-06-10
影响因子：
2.6
通讯作者：
Gu, Z. M.
中科院分区：
文献类型：
--
作者：
Liu, Y.;Whipps, C. M.;Gu, Z. M.
kitauei Egusa et Nakajima， 1981年从日本鲤鱼鲤鲤（Cyprinus carpio L）中发现。在中国，一种感染同一寄主肠道组织的粘孢子虫被描述为Thelohanellus xinyangensis Xie， Gong, Xiao, Guo, Li et Guo, 2000，尽管与T. kitauei有许多相似之处。为了研究这些物种的同质性，我们对信阳赤潮进行了形态学和分子生物学的研究。比较粘孢子形态，各种孢子的平均长度和宽度在种间并不相同，但维数范围重叠。这些数据更能说明种内变异，而不是不同的物种。比较两种孢子的孢子长与极蒴果长、孢子宽与极蒴果宽的相对比值无差异，扫描电镜显示，信阳孢子表面光滑，与kitauei一致。最令人信服的是，这两个物种的小亚基核糖体RNA （ssrRNA）基因的DNA序列是相同的。从形态学和分子生物学的角度来看，我们认为普通鲤鱼肠中的信阳T. xinyangensis不是一个独立的物种，与T. kitauei同义。(C) 2011 Elsevier B.V.版权所有
Thelohanellus kitauei Egusa et Nakajima, 1981, was described from common carp Cyprinus carpio L in Japan. In China, a myxosporean infecting the intestinal tissue of the same host species was described as Thelohanellus xinyangensis Xie, Gong, Xiao, Guo, Li et Guo, 2000, despite many similarities to T. kitauei. To examine the potential conspecificity of these species, a morphological and molecular investigation of T. xinyangensis was conducted. Comparing myxospore morphology, the mean spore length and width of each species is not identical between species, but ranges of dimensions overlap. These data are more suggestive of intraspecific variation than distinct species. Comparison of relative ratios of spore length to polar capsule length and spore width to polar capsule width of T. xinyangensis and T. kitauei reveal no differences and scanning electron microscopy reveals a smooth spore surface of T. xinyangensis, which is consistent with that of T. kitauei. Most convincingly, DNA sequences of the small subunit ribosomal RNA (ssrRNA) gene of the two species were identical. From the morphological and molecular biological data, we propose T. xinyangensis from the intestine of common carp is not a distinct species and is synonymous with T. kitauei. (C) 2011 Elsevier B.V. All rights reserved.