EFFECT OF PLANT AND MICROBIAL CHITINASES ON THE EGGS AND JUVENILES OF MELOIDOGYNE-HAPLA CHITWOOD (NEMATODA, TYLENCHIDA)
EFFECT OF PLANT AND MICROBIAL CHITINASES ON THE EGGS AND JUVENILES OF MELOIDOGYNE-HAPLA CHITWOOD (NEMATODA, TYLENCHIDA)
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DOI：
10.1163/187529292x00199
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发表时间：
1992-04-01
期刊：
影响因子：
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通讯作者：
GRANT, JL
中科院分区：
文献类型：
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作者：
MERCER, CF;GREENWOOD, DR;GRANT, JL
比较了菜豆、粘质沙雷氏菌和灰色链霉菌的几丁质酶对哈普拉根结线虫卵和幼虫的影响。三种来源的几丁质酶均能显著提高线虫卵的孵化率。在粘质链霉菌和灰链霉菌的几丁质酶处理后，许多卵保持球形，伴随而来的是死亡幼虫数量的增加。纯化的几丁质酶在17-mU-g蛋白/毫升及以上的浓度下观察虫卵的死亡率。结果表明，外源几丁质酶可导致线虫卵提前孵化，可作为线虫防治的辅助手段。
Chitinases from Phaseolus vulgaris, Serratia marcescens and Streptomyces griseus were compared for their effects on eggs and juveniles of Meloidogyne hapla Chitwood. Increased hatch rates of nematode eggs were observed on incubation with chitinase from all three sources. After treatment with chitinases from S. marcescens and St. griseus many of the eggs remaining spherical with a concomitant increase in the number of dead juveniles. Egg mortality observed with purified P. vulgaris chitinase at concentrations of 17-mu-g protein/ml and above. The results suggest that exogenous chitinases cause premature hatch of nematode eggs and could be used as an aid in the control of nematodes.