Tenocyte proliferation and migration promoted by rat bone marrow mesenchymal stem cell-derived conditioned medium
Tenocyte proliferation and migration promoted by rat bone marrow mesenchymal stem cell-derived conditioned medium
复制标题
大鼠骨髓间充质干细胞源条件培养基促进肌腱细胞增殖和迁移
DOI：
10.1007/s10529-017-2446-7
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发表时间：
2018-01-01
影响因子：
2.7
通讯作者：
Song, Guanbin
中科院分区：
文献类型：
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作者：
Chen, Qiufang;Liang, Qingfei;Song, Guanbin
探讨大鼠骨髓间充质干细胞（MSCs）分泌因子对肌腱细胞增殖和迁移的影响，为开发基于MSCs的肌腱损伤治疗方法提供依据。大鼠骨髓间充质干细胞衍生条件培养基（MSC-CM）能促进大鼠骨髓间充质干细胞在24 h内的增殖，降低G1期小细胞的比例。MSC-CM激活细胞外信号调节的激酶1/2 （ERK1/2）信号分子，而ERK1/2抑制剂PD98059消除了MSC-CM诱导的细胞增殖，降低了G1期的细胞比例，提高了p-ERK1/2的表达。此外，MSC-CM促进了6 h内肌腱细胞的迁移，增强了丝状肌动蛋白（F-actin）的形成，增加了肌腱细胞的细胞和核刚度。MSC-CM通过ERK1/2信号通路改变细胞周期分布，促进细胞增殖。msc - cm诱导的小细胞迁移伴随着细胞骨架聚合和细胞和核刚度的增加。
To investigate the impact of secreted factors of rat bone marrow mesenchymal stem cells (MSCs) on the proliferation and migration of tenocytes and provide evidence for the development of MSC-based therapeutic methods of tendon injury.Rat bone marrow mesenchymal stem cell-derived conditioned medium (MSC-CM) promoted the proliferation of tenocytes within 24 h and decreased the percentage of tenocytes in G1 phase. MSC-CM activated the extracellular signal-regulated kinase1/2 (ERK1/2) signal molecules, while the ERK1/2 inhibitor PD98059 abrogated the MSC-CM-induced proliferation of tenocytes, decreased the fraction of tenocytes in the G1 phase and elevated p-ERK1/2 expression. Furthermore, MSC-CM promoted the migration of tenocytes within 6 h, enhanced the formation of filamentous actin (F-actin) and increased the cellular and nuclear stiffness of tenocytes.MSC-CM promotes tenocyte proliferation by changing cell cycle distribution via the ERK1/2 signaling pathway. MSC-CM-induced tenocyte migration was accompanied by cytoskeletal polymerization and increases in cellular and nuclear stiffness.